Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Immunological Characterization of Vibrio vulnificus isolated from Marine Environment

해양에서 분리한 Vibrio vulnificus의 면역학적 특성

  • Jung, Cho-Rok (Division of Biological Sciences, Ulsan University) ;
  • Jeon, You-Jin (Faculty of Applied Marine Sciences, Cheju National University) ;
  • Heo, Moon-Soo (Faculty of Applied Marine Sciences, Cheju National University)
  • 정초록 (울산대학교 생명과학부) ;
  • 전유진 (제주대학교 해양생산과학부) ;
  • 허문수 (제주대학교 해양생산과학부)
  • Published : 2001.12.24
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Abstract

Immunoglobulin G was purified by 40% $(NH_4)_2SO_4$ precipitation, DEAE-Sephadex, Sephadex G-150 column chromatographies from rabbit antiserum against V. vulnificus ATCC 27562 O antigen and used for immunological test for V. vulnificus isolates. The profiles of cell lysate total protein and outer membrane protein from the isolates were analyzed by SDS-PAGE and densitometry. The overall profiles in all isolates were similar. Distict protein band was observed in comparison with V. parahaemolyticus. Western Blotting with rabbit Immunoglobulin G against cell lysates and OMP of V. vulnificus isolates showed a strong antigenic response to antigen 66, 60, 54, 48, 33 and 26 kDa which were common to all strains examined. The 26 kDa antigen showed V. vulnificus specific antigen in comparison with Vibrio parahaemolyticus. A sandwich enzyme-linked immunosorbent assay was developed by using rat anti-V. vulnificus ATCC 27562 polyclonal antibodies as capture antibody, a purified rabbit IgG antibody as detector antibody, and goat anti-rabbit IgG-alkaline phosphatase conjugate as developer antibody. When four V. vulnificus isolates were tested, the reactivity showed from 50 to 70% by sandwich ELISA.

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