Maxillofacial Plastic and Reconstructive Surgery
- 제23권4호
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- Pages.295-305
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- 2001
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- 2288-8101(pISSN)
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- 2288-8586(eISSN)
화학적 발암화에 따른 Protein Kinase C의 발현 변화
EXPRESSION OF PROTEIN KINASE C ISOFORMS IN CHEMICAL CARCINOGEN-INDUCED NEOPLASTIC TRANSFORMATION OF HUMAN EPITHELIAL CELLS
- Byeon, Ki-Jeong (Dept. of Dentistry, Ulsan University Hospital) ;
- Hong, Lak-Won (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Kyungpook National University) ;
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Kim, Chin-Soo
(Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Kyungpook National University)
- 발행 : 2001.08.30
초록
구강은 흡연이나 음주와 같은 화학적 발암물질이 쉽게 접촉할 수 있는 화학적 발암물질의 표적장기이며 구강암을 포함한 대부분의 암 발생의 근원이 되는 세포는 상피세포이다. 따라서 본 연구는 인체상피세포를 화학적 발암물질인 MNING에 노출시켜 발암화를 유도하고 이에 따른 작용 기전을 분석함으로써 구강암과 같은 상피세포 기원의 종양 발생기전을 이해하는 데 기여하고자 하였다. 인체 상피세포에
Protein kinase C (PKC) is known to play a pivotal role in neoplastic transformation cells and its high expression is often found in a variety of types of tumors including oral cancer. While PKC is associated with the altered signal transduction pathway of the tumor cells, it is still unclear which isoform is involved in the carcinogenesis process. Since the cellular distributions and the roles of PKC are isoform-specific, it is very important to identify the specific target molecules to improve our understanding of the carcinogenesis processes. Thus, the present study attempted to perform chemical carcinogen-induced neoplastic transformation of human epithelial cells and analyze the specific isoform of PKCs involved in the cellular transformation. The study analyzed overall PKC responses upon MNNG(N-Methyl-N'-nitro-N-nitroso guanidine) exposure with [