Clinical and Experimental Reproductive Medicine
- Volume 28 Issue 2
- /
- Pages.161-167
- /
- 2001
- /
- 2233-8233(pISSN)
- /
- 2233-8241(eISSN)
The Effect of GM-CSF Supplementation in Culture Medium in the Human IVF Programs
체외수정 시술시 배양액에 첨가된 과립구 대식세포 증식인자 (Granulocyte-Macrophage Colony Stimulating Factor)의 효과
- Park, Won-Il (Department of OB/GYN, Eulji University School of Medicine) ;
- Kwon, Hynck-Chan (Department of OB/GYN, Eulji University School of Medicine) ;
- Kim, Dong-Hoon (Medical Science Institute, Eulji General Hospital) ;
- Kang, Hee-Kyoo (Medical Science Institute, Eulji General Hospital) ;
- Kim, Myo-Kyung (Medical Science Institute, Eulji General Hospital) ;
- Lee, Hoi-Chang (Medical Science Institute, Eulji General Hospital) ;
- Jung, Ji-Hak (Department of OB/GYN, Eulji University School of Medicine) ;
- Lee, Myong-Seop (Department of OB/GYN, Eulji University School of Medicine) ;
- Lee, Ho-Joon (Department of Physiology, Eulji University School of Medicine)
- 박원일 (을지의과대학교 산부인과) ;
- 권혁찬 (을지의과대학교 산부인과) ;
- 김동훈 (을지병원 의과학연구소) ;
- 강희규 (을지병원 의과학연구소) ;
- 김묘경 (을지병원 의과학연구소) ;
- 이회창 (을지병원 의과학연구소) ;
- 정지학 (을지의과대학교 산부인과) ;
- 이명섭 (을지의과대학교 산부인과) ;
- 이호준 (을지의과대학교 생리학교실)
- Published : 2001.06.30
Abstract
Objective: Granulocyte-macrophage colony stimulating factors known to be secreted in murine and human reproductive tract. The development of human, bovine and murine embryos could be promoted by addition of GM-CSF in culture medium. However, the pregnancy and implantation rate of embryos cultured in GM-CSF have not been evaluated. The aim of this study was to assess the effect of GM-CSF in embryo development, pregnancy and implantation rate. Methods: A total of 191 IVF cycles were divided into control and GM-CSF supplement group (control=96, GM-CSF=95). The embryos were cultured for three day with or without 2 ng/ml of recombinant human GM-CSF. The quality of embryo, developmental velocity, pregnancy and implantation rates were compared. Results: There was no difference in age, number of gonadotropin ampules used, number of oocytes and fertilization. The number of ICSI cycle was higher in GM-CSF group. In GM-CSF group, G-1 grade embryos were the highest in proportion (56.4%), while G-2 grade embryos were highest (44.3%) in control group. The developmental velocity of embryos were not different between GM-CSF and control group. The pregnancy and implantation rates were significantly higher in GM-CSF group than control (47.4% vs. 33.3%, 17.0% vs. 11.1% respectively). Conclusion: By adding GM-CSF in culture medium, the quality of embryo, pregnancy and implantation rate could be improved.