Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Effects of Heparin-Binding Epidermal Growth Factor on the Expression of MMP-9 and ATPase ${\gamma}$-subunit mRNA in the Mouse Embryo

생쥐 배아에서 Heparin-Binding Epidermal Growth Factor가 MMP-9과 ATPase ${\gamma}$-subunit 유전자의 발현에 미치는 영향

  • Hong, Seok-Ho (Department of Biology, College of Natural Sciences, Hanyang University) ;
  • Nah, Hee-Young (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center) ;
  • Lee, Ji-Yun (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center) ;
  • Kim, Jung-Hee (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center) ;
  • Kim, Chung-Hoon (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center) ;
  • Chae, Hee-Dong (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center) ;
  • Kang, Byung-Moon (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center) ;
  • Kim, Moon-Kyoo (Department of Biology, College of Natural Sciences, Hanyang University)
  • 홍석호 (한양대학교 자연과학대학 생명과학과) ;
  • 나희영 (울산대학교 의과대학 서울중앙병원 산부인과학교실) ;
  • 이지윤 (울산대학교 의과대학 서울중앙병원 산부인과학교실) ;
  • 김정희 (울산대학교 의과대학 서울중앙병원 산부인과학교실) ;
  • 김정훈 (울산대학교 의과대학 서울중앙병원 산부인과학교실) ;
  • 채희동 (울산대학교 의과대학 서울중앙병원 산부인과학교실) ;
  • 강병문 (울산대학교 의과대학 서울중앙병원 산부인과학교실) ;
  • 김문규 (한양대학교 자연과학대학 생명과학과)
  • Published : 2001.06.30
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Abstract

Objectives: The purpose of this study was to evaluate effects of heparin-binding epidermal growth factor (HB-EGF) on the rate of blastocyst formation and hatching in the mouse embryos and the expression of matrix metalloproteinase-9 (MMP-9) and ATPase ${\gamma}$-subunit mRNA. Methods: Late 2-cell mouse embryos was cultured for 72 hours in RTF medium containing with 1, 10, and 100 ng/ml HB-EGF. The mRNA expression level of MMP-9 and ATPase ${\gamma}$-subunit was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results: The rate of hatching was significantly higher (p<0.05) in group containing with 1 ng/ml HB-EGF than other groups. Also, the rate of hatched blastocyst was significantly higher (p<0.05) in 10 ng/ml. The mRNA expression level of MMP-9 mRNA was not shown any difference among groups, but ATPase ${\gamma}$-subunit was higher than other groups. Conclusions: Taken together these results suggest that HB-EGF has the positive effect to promote the blastocyst formation and hatching process and influences the blastocoel expansion by promoting the ATPase mRNA expression in the mouse embryos.

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