JSTS:Journal of Semiconductor Technology and Science

The Institute of Electronics and Information Engineers (대한전자공학회)
권호 표지

A Photosensitive Glass Chip for DNA Purification of Nucleic Acid Probe Assay

  • Kim, Joon-Ho (Department of Electrical Engineering and Computer Science(Divison of E.E), Korea Advanced Institute of Science and Technology(KAIST)) ;
  • Kim, Byung-Gyun (Department of Electrical Engineering and Computer Science(Divison of E.E), Korea Advanced Institute of Science and Technology(KAIST)) ;
  • Yoon, Jun-Bo (Department of Electrical Engineering and Computer Science(Divison of E.E), Korea Advanced Institute of Science and Technology(KAIST)) ;
  • Euisik Yoon (Department of Electrical Engineering and Computer Science(Divison of E.E), Korea Advanced Institute of Science and Technology(KAIST))
  • Published : 2001.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

A new DNA purification chip is proposed and fabricated for the sample preparation of Nucleic Acid (NA) probe assay. The proposed DNA purification chip is fabricated using photosensitive glass substrate and polydimethylsiloxane (PDMS) cover fixture. We have successfully captured and eluted the DNA using the fabricated photosensitive glass chip. The fabricated DNA purification chip showed a binding capacity of $15ng/\textrm{cm}^2$and a minimum extractable input concentration of $100copies/200\muL$. The proposed DNA purification chip can be applied for low-cost, disposable sample preparation of NA probe assays.

Keywords

References

  1. J. Buitkamp and J. T. Epplen, 'Modern genome research and DNA diagnostics in domestic animals in the light of classical breeding techniques,' Electrophoresis, vol. 17, pp. 1-11, 1996 https://doi.org/10.1002/elps.1150170102
  2. H. C. Hoch, L. W. Jelinski and H. G. Craighead, 'Nanofabrication and biosystems,' New York, Cambridge University Press, 1996
  3. C. H. Mastrangelo, M. A. Burns and D. T. Burke, 'Microfabricated Devices for Genetic Diagnostics,' Proc. of The IEEE, vol. 86, No.8, pp. 1769-1787, 1998 https://doi.org/10.1109/5.704282
  4. L. A. Christel, K. Petersen, W. Mcmillan, M. A. Northrup, 'Rapid, Automated Nucleic Acid Probe Assays Using Silicon Microstructures for Nucleic Acid Concentration,' Journal of Biomechanical Engineering, vol. 121, pp. 22-27, Feb. 1999
  5. A. Manz, H. Becker, 'Micro system technology in chemistry and life science,' Berlin: Springer-Verlag, 1998
  6. W. R. Boom, H. M. A. Adriaanse, T. Kievits, T. Hague, P. F. Lens, 'Process for isolating nucleic acid,' US patent No. 5,234,809, 1993
  7. P. Belgrader, R. Joshi, J. Ching, S. Zaner, D. A. Brokholer, and M. A. Northrup, 'Real-time PCR analysis on Nucleic acids purified from plasma using silicon chip,' Proc. ${\mu}TAS2000$ Netherlands, pp.525-528, 2000
  8. K. A. Melzak, C. S. Sherwood, R. F. B. Turner and C. A. Haynes, 'Driving forces for DNA adsorption to silica in perchlorate solutions,' Journal of Colloid and Interface Science, Vol. 181, pp. 635-644, 1996 https://doi.org/10.1006/jcis.1996.0421
  9. R. Lakshmi, V. Baskar, U. Ranga, 'Extraction of Superior-Quality Plasmid DNA by a Combination of Modified Alkaline Lysis and Silica Matrix,' Analytical Biochemistry, vol.271, pp. 109-112, 1999 https://doi.org/10.1006/abio.1999.4125
  10. Y.-R. Cho, J-Y. Oh, H.-S. Kim, and H.-S. Jeong, 'Micro-etching technology of high aspect ratio framworks for electronic devices.' Material science and Engineering, vol. B, No. 64, pp.79-83, 1999 https://doi.org/10.1016/S0921-5107(99)00158-0
  11. K. Feindt, A. Harnisch, V. Zopping, D. Hulsenberg, and E. Kallenbach, '3-D Structuring of Photosensitive glasses,' Proc. MEMS'98 confrence, Heidelberg, Germany, pp.207-210, 1998
  12. http://www.mikroglas.com