Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of Artificial Soils and Aqueous Solutions for Plantlet Acclimatization of Somatic Embryos of Aralia elata

두릅나무 체세포배 유래 소식물체의 순화에 미치는 배양토 및 공급액의 효과

  • Published : 2001.09.01
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Abstract

In order to develop effective acclimatization methods for Aralia elata plantlets regenerated from somatic embryos, various acclimatizing conditions were compared regarding both survival rate and growth of the plantlets. The plantlets were transplanted into plastic boxes containing artificial soil in the presence of either several levels of MS liquid media, distilled water, 2% sucrose or 0.1% hyponex solution. They were then cultured by spraying of distilled water twice a week and maintained in the normal tissue culture room. Perlite was proved to be better than vermiculite on survival rate and growth of the plantlets. As the size of perlite (larger than 0.2 cm in diameter) increased, both the survival rate and growth of the plantlets improved. Among the various MS liquid media and different aqueous solutions tested, distilled water appeared to result in the best survival rate and growth. MS media were also effective in increasing survival rate and supporting growth when diluted to 1/4 and/or 1/8. The acclimatized plantlets could be transplanted directly onto the nursery bed and grown normally. The above results suggest that plantlets regenerated from somatic embryos of Aralia elata be effectively acclimatized using a plastic box containing perlite with distilled water treatment.

두릅나무의 체세포배 유래 소식물체의 효과적인 순화 방법을 개발하고자 플라스틱 용기를 사용 4가지 인공배양토 및 MS 액체배지 등 7가지 수용액 처리를 통해 활착률 및 생장을 조사하였다. 상토는 perlite가 vermiculite보다 활착 및 생장에 양호하였고, 입자가 클수록 활착률 및 생장이 좋았다. 액체배지는 2차 증류수 처리가 가장 양호하여 96%의 활착률을 나타냈으며, 염류농도를 1/4 및 1/8로 낮춘 MS배지는 각각 92%의 활착률을 보인 반면 기본배지 및 1/2 MS배지는 활착률이 저조하였다. 한편 0.1% hyponex와 2% sucrose 처리는 84% 및 76%의 활착률을 각각 나타냈다. 결론적으로 두릅나무의 체세포배 유래 소식물체의 순화는 입자가 큰 perlite를 사용하고 증류수 처리 혹은 염류 농도를 낮춘 MS배지 처리로 효과적인 순화가 가능함을 보여주었다.

Keywords

References

  1. Automation and Evironmental Control in Plant Tissue Culture Automation on plant tissue culture - general introduction and overview Aitken-Christie J;T Kozai;S Takayama;J Aitken-Christie(ed.);T. Kozai(ed.);M. Lila Smith(ed.)
  2. Cell and Tissue Culture in Forestry v.2 Nursery handling of propagules Driver JA;GRL Suttle;Bonga(ed.);Durzan(ed.)
  3. In Practice v.2 Plant propagation by tissue culture George EF
  4. Plant Cell Tiss Org Cult v.22 One step shoot tip multuplication and rooting of Digitalis thapsi L Herrera MT;M Cacho;MP Corchete;J Fernandez-Tarrago
  5. Kor J Plant Tiss Cult v.21 Somatic embryogenesis and plant regeneration in suspension culteres of Aralia elata Jhang HH;Park CH;Lee YS;Shin YB
  6. Res Rep Inst For Gen Kor v.26 In vivo rooting of shoots propagated by bud culture of Juglans Kwon YJ;Youn Y;Lee SK;Hyun YI;Lee JJ;Lee MH
  7. Environ Cont Biol v.33 Effects of $CO_2$ enrichment and supporting material on growth, photosynthesis and water potential of Eucalyptus shoots/plantlets cultured photoautographically in vitro Kirdmanee C;Y Kitaya;Y Kosai
  8. In Vitro Cell Dev Biol v.27 Photoautotrophic micropropagation Kozai T
  9. Can J Plant Sci v.72 A transplant plug technique for production of alfalfa (Medicago sativa L.) plants form somatic embryos McElroy AR;DCW Brown
  10. Kor J Plant Tiss Cult v.28 Genotype effect on somatic embryogenesis and plant regeneration of 15 Aralia elata Moon HK;Hong YP;Kim YW;Lee JS
  11. Somatic Embryogenesis in Woody Plants v.5 Somatic Embryogenesis from winter buds of 10-year-old Aralia elata Moon HK;Youn Y;Jain SM(ed.);Gupta PK(ed.);Newton RJ(ed.)
  12. J Kor For Soc v.82 In vitro shoot proliferation by pulse treatment from shoot culteres of Q.acutissima and ex vitro root induction using peat plug systems in Quercus spp Moon HK;Youn Y;Son SH;Lee SK;Yi JS
  13. Physiol Plant v.15 A revised medium for rapid growth and bioassays with tobacco tissue culture Murashige T;F Skoog
  14. S.Kor J Med Crop Sci v.2 Effects of media and plant growth regulators on germination of somatic embryos of Aralia elata Park CH;Lee YS;Jhang HH;Kim NS;Shin YB
  15. In vitro culture of higher plants Pierik RLM
  16. Automation and Environmental Control in Plant Tissue Culture Commercialization of tissue culture and automated systems Wilson K;J Aitken-Christie(ed.);T Kozai(ed.);M Lila Smith(ed.)
  17. Biotechnology in Agriculture and Forestry v.19 Micropropagation of Cucumis Ziv M;YPS Bajaj(ed.)
  18. Automation and Environmental Control in Plant Tissue Culture In Vitro acclimatization Ziv M;J Aitken-Christie(ed.);T Kozai(ed.);M Lila Smith(ed.)