Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Isolation and Phylogenetic Analysis of Acyl-CoA-binding Protein Gene from Panax ginseng C.A. Meyer

인삼으로부터 Acyl-CoA-binding Protein 유전자의 동정 및 계통적 분석

  • 인준교 (한국인삼연초연구원 신사업연구부) ;
  • 류명현 (한국인삼연초연구원 신사업연구부) ;
  • 최광태 (한국인삼연초연구원 신사업연구부) ;
  • 최관삼 (충남대학교 응용생물화학부) ;
  • 김세영 (경희대학교 농학과) ;
  • 양덕춘 (한국인삼연초연구원 신사업연구부)
  • Published : 2001.07.01
Download PDF
⟨ Previous Next ⟩

Abstract

Acyl-CoA binding proteins (ACBP) are small highly-conserved cytosolic proteins that bind long-chain acyl-CoAs. A cDNA encoding ACBP was identified from cDNA library constructed from hairy root poly $A^{＋}$ RNA in expressed sequence tags (EST) analysis. The cDNA clone was 453 bp long and carried an open reading frame of 264 bp (10 kDa). The ginseng ACBP amino acid sequence was compared with other reported plant ACBPs using the CLUSTALW. Ginseng ACBP is 89%, 81%, 80%, and 73% identical with ACBP from castor bean, lilly, Digitalis and Arabidopsis, respectively. However, ginseng ACBP is 5 amino acids smaller than Arabidopsis and rape seed ACBPs. Also there is no any known signal peptide sequence in ginseng ACBP.

Acyl-binding protein (ACBP)은 긴사슬 acyl-CoA와 결합하는 고도로 보존되어 있는 세포질 단백질이다. 인삼의 유용 유전자를 대량으로 분석하기 위하여 제작된 인삼 모상근cDNA library로부터 인삼 ACBP유전자가 분리되었다. 이 유전자는 길이가 453 bp이고 264 bp의 open reading frame (10kDa)을 가지고 있었다. 인삼 ACBP의 아미노산 서열을 다른 식물체에서 보고된 것과 비교한 결과 castor bean과 89.5%로 매우 높은 유사성을 나타내었으며, lilly, Digitalis. Arabidopsis, rape 등과 각각 81.8%, 80.7%, 73%, 71.9%의 유사성을 나타내었다. 그러나 인삼의 ACBP는 Arabidopsis 와 rape의 ACBP보다 5개의 아미노산이 적은 87개의 아미노산으로 이루어져 있었고 어떠한 signal peptide로 발견되지 않았다. 그리고 현재 보고되어 있는 다른 식물체의 ACBP와 계통분석을 한 결과 인삼의 ACBP는 Arabidopsis나 cotton 보다는 castor bean과 매우 가까운 유연관계에 있는 것으로 나타났다.

Keywords

References

  1. Physiol Plant v.100 Lipid-transfer proteins: tools for manipulating membrane lipids Bourgis, F;Kader, J. C
  2. Plant Mol Biol v.38 Arabidopsis cDNA encoding a membrane-associated protein with an acyl-CoA binding domain Chye, M. L
  3. Arch Biochem Biophys v.331 Characterization of an acyl-CoA-binding protein from Arabidopsis thaliana Engeseth, N. J;Pacovsky, R. S;Newman, T;Ohlrogge, J
  4. Plant Physiol v.114 A cDNA Clone for Acyl-CoA-binding Protein from Castor Bean (Accession No. Y08996)(PGR97-075) Erber, A;Horstmann. C;Schobert, C
  5. Biochem J v.323 Role of long-chain fatty acyl-CoA esters in the regulation of metabolism and in cell signalling Faergemann, N. J;Knudsen, J
  6. Plant Molecular Biology v.25 Molecular cloning of a cDNA from Brassica napus L. for a homologue of acyl-CoA-binding protein Hills, M. J;Dann, R;Lydiate, D. L;Sharpe, A
  7. Proc Natl Acad Sci USA v.83 Putative diazepam binding inhibitor peptide: cDNA clones from rat Mocchetti, E;Einstein, R;Brosius, J
  8. Biochem J v.241 A novel acyl-CoA-binding protein from bovine liver Mogensen, I. B;Schulenberg, H;Hansen, H. O;Spener, F;Knudsen, J
  9. Biochem J v.299 Acyl-CoA-binding protein (ACBP) can mediate intermembrane acyl-CoA transport and donate acyl-CoA for beta-oxidation and glycerolipid synthesis Rasmussen, J. T;Faergemann, N. J;Kristiansen, K;Knudsen, J
  10. Plant Physiol v.111 A cDNA Encoding Acyl-CoA-binding Protein from Cotton (U35015).(PGR96-028) Reddy, A. S;Ranganathan, B;Haisler, R. M;Swize, M. A
  11. Planta v.162 Molecular cloning and characterisation of DNAs complementary to mRNAa from wounded potato (Solanum tuberosum) tubertissue Shirras, A. D;Northcote, D. H
  12. DNA v.6 Bovine and human cDNA sequences encoding a putative benzodiazepine recepine receptor ligand Webb, N. R;Rose, T. M;Malik, N;Marquardt, H;Shoyab, M;Todaro, G. J;Lee, D. C