Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Marker Genes for in Vitro Selection of Transgenic Plants

  • Brasileiro, Ana C.M. (Embrapa Recursos Geneticos e Biotecnologia, Parque Estacao Biologica(PqEB), Brasil) ;
  • Aragao, Francisco J.L. (Embrapa Recursos Geneticos e Biotecnologia, Parque Estacao Biologica(PqEB), Brasil)
  • Published : 2001.09.01
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Abstract

The use of a marker gene in a transformation process aims to give a selective advantage to the transformed cells, allowing them to grow faster and better, and to kill the non-transformed cells. In general, the selective gene is introduced into plant genome along with the genes of interest. In some cases, the marker gene can be the gene of interest that will confer an agronomic characteristic, such as herbicide resistance. In this review we list and discuss the use of the most common selective marker genes on plant transformation and the effects of their respective selective agents. These genes could be divided in categories according their mode of action: genes that confer resistance to antibiotics and herbicides; and genes for positive selection. The contention of the marker gene flow through chloroplast transformation is further discussed. Moreover, strategies to recover marker-free transgenic plants, involving multi-auto-transformation (MAT), co-transformation, site specific recombination and intragenomic relocation of transgenes through transposable elements, are also reviewed.

Keywords

References

  1. Theor Appl Genet v.93 Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.)co-transformed via particle bombardment Aragao FJL;Barros LMG;Brasileiro ACM;Ribeiro SG;Smith FD;Sanford JC;Faria JC;Rech EL
  2. Theor Appl Genet v.101 Selection of transgenic meristematic cells utilizing a herbicidal molecule results in the recovery of fertile transgenic soybean [Glycine max (L.) Merril] Merril] plants at high frequency Aragao FJL;Sarokin L;Vianna GR;Rech EL
  3. Annu Rev Biochem v.42 Mechanisms of antibiotic resistance in bacteria Benveniste R;Davies J
  4. Nature v.304 A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation Bevan MW;Flavell RB;Chilton MD
  5. Trends Biotech v.16 Engineering chloroplasts: an alternative site for foreign genes, proteins, reactions and products Bogorad L
  6. Transgenic plants Markers for plant gene transfer Bowen BA;Kung, S(ed.);Wu, R(eds.)
  7. Manual de transformacao genetica de plantas Neomicina Fosfotransferase Ⅱ (NPT Ⅱ) Brasileiro ACM;Brasileiro, A. C. M(ed.);Carneiro, V. T. C(eds.)
  8. Cultura de tecidos e transformacao genetica de plantas Transformacao genetica de plantas Brasileiro ACM;Dusi DMA;Torres, A.C(ed.);Caldas L. S(ed.);Buso J.A(eds.)
  9. Transg Res v.1 Expression of the mutant Arabidopsis thaliana acetolactate synthase gene confers chlorsulfuron resistance to transgenic poplar plants Brasileiro ACM;Tourneur C;Leple JC;Combes V;Jouanin L
  10. Science v.223 Herbicide-resistant mutants from tobacco cell cultures Chaleff RS;Mauvais CJ
  11. Nature v.317 Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate Comai L;Facciotti D;Hiatt WR;Thompson G;Rose RE;Stalker DM
  12. Science v.221 An altered aroA gene product confers resistance to the herbicide glyphosate Comai L;Sen LC;Stalker DM
  13. Plant Cell Rep v.17 Co-transformation with one Agrobacterium tumefaciens strain containing two binary plasmid as a method for producing marker-free transgenic plants Daley M;Knauf VC;Summerfelt KR;Turner JC
  14. Nature Biotech v.16 Containment of herbicide resistance through genetic engineering of the chloroplast genome Daniell H;Datta R;Varma S;Gray S;Lee SB
  15. EMBO J v.6 Engeneering herbicide resistance in plants by expression of a detoxifying enzyme De Block M;Botterman J;Vanderwiele M;Dockx J;Thoen C;Gossele V;Rao Movva N;Thompson C;Van Montagu M;Leemans J
  16. Theor Appl Genet v.82 Two T-DNAs co-transformed into Brassica napus by a double Agrobacterium infection are mainly integrated at the same locus De Block M;Debrouwer D
  17. Proc Natl Acad Sci USA v.94 Selection of marker-free transgenic plants using the isopentenyl transferase gene Ebinuma H;Sugita K;Matsunaga E;Yamakado M
  18. Proc Natl Acad Sci USA v.80 Expression of bacterial genes in plant cells Fraley RT;Rogers SG;Horsch RB;Sanders PR;Flick JS;Adams SP;Bittner ML;Brand LA;Fink CL;Fry JS;Galluppi GR;Goldberg SB;Hoffmann NL;Woo SC
  19. Plant Mol Biol v.39 Positive-negative selection and T-DNA stability in Arabidopsis transformation Gallegeo ME;Sirand-Pugnet P;White CI
  20. Plant Mol Biol v.40 Selectable marker-free transgenic plants without sexual crossing: transient expression of cre recombinase and use of a conditional lethal dominant gene Gleave AP;Mitra DS;Mudge SR;Morris BAM
  21. Bio/Technology v.11 Transposition mediated repositioning and subsequent elimination of marker genes from transgenic tomato Goldsbrough AP;Lastrella CN;Yoder JI
  22. Plant Mol Biol v.37 The xylose isomerase gene from Thermoanaerobacterium thermosulfurogenes allows effective selection of transgenic plant cells using D-xylose as the selection agent Haldrup A;Petersen SG;Okkels FT
  23. Plant Cell Rep v.18 Positive selection: a plant selection principle based on xylose isomerase, an enzyme used in the food industry Haldrup A;Petersen SG;Okkels FT
  24. Mol Gen Genet v.211 Transformation with a mutant Arabidopsis acetolactate syntase gene renders tobacco resistant to sulfonylurea herbicides Haughn GW;Smith J;Mazur B;Somerville C
  25. EMBO J v.2 Chimeric genes as dominant selectable markers in plant cells Herrera-Estrella L;De Block M;Messens E;Hernalsteens JP;Van Montagu M;Schell J
  26. Plant J v.16 Transient expression of green fluorescent protein in various plastid types following microprojectile bombardment Hibberd JM;Linley PJ;Khan MS;Gray JC
  27. Mol Breed v.4 Analysis of mannose selection used for transformation of sugar beet Joersbo M;Donaldson I;Kreiberg J;Petersen SG;Brunstedt J;Okkels FT
  28. Mol Breed v.6 Relationship between promoter strength and transformation frequencies using mannose selection for the production of transgenic sugar beet Joersbo M;Mikkelsen JD;Brunstedt j
  29. Plant Cell Rep v.16 A novel principle for selection of transgenic plant cells: positive selection Joersbo M;Okkels FT
  30. Physiol Plant v.105 Parameters interacting with mannose selection employed for the production of transgenic sugar beet Joersbo M;Petersen SG;Okkels FT
  31. Genetics v.152 Homeologous plastid DNA transformation in tobacco is mediated by multiple recombination events Kavanagh TA;Thanh ND;Lao NT;Mcgrath N;Peter SO;Horvath EM;Dix PJ;Medgyesy P
  32. Nature Biotech v.17 Fluorescent antibiotic resistance marker for tracking plastid transformation in higher plants Khan MS;Maliga P
  33. In Vitro Cell Dev Biol Plant v.34 PEG-mediated plastid transformation in higher plants Kofer W;Eibl C;Steinmuller K;Koop HU
  34. Nature Biotech v.17 Inducible isopentenyl transferase as a high-efficiency marker for plant transformation Kunkel T;Niu QW;Chan YS;Chua NH
  35. J. Biotech v.26 Genetic manipulation of crop plants Lindsey K
  36. Annu Rev Plant Physiol v.40 The development of herbicide resistant crops Mazur BJ;Falco SC
  37. Biol Chem v.380 Prospects for the precise engineering of plant genomes by homologous recombination Mengiste T;Paszkowski J
  38. Planta v.196 A double mutant allele, crs1-4, of Arabidopsis thaliana encodes an acetolactase synthase with altered kinetics Mourad G;Williams D;King J
  39. Mol Gen Genet v.205 The bialaphos biosynthetic genes of Streptomyces hygroscopicus:molecular cloning and characterization of the gene cluster Murakami T;Anzai H;Imai S;Satoh A;Nagaoka K;Thompson CJ
  40. 8th Biennial Conference of the Cellular and Molecular Biology of the Soybvean Murh C;Gacon F;Betka S;Le-Roux S;Lestrade B;Portelatine L;Sailland A;Pelissier B
  41. Plant Cell Rep v.19 The use of phosphomannose-isomerase as a selectable marker to recover transgenic maize plants (Zea mays L.)via Agrobacterium transformation Negrotto D;Jolley M;Beer S;Wenck AR;Hansen G
  42. J Amer Soc Hort Sci v.118 The role of aminoglycoside antibiotics in the regeneration and selection of neomycin phosphotransferase-transgenic apple tissue Norelli JL;Aldwinckle HS
  43. Phytochemistry v.46 Synthesis of cytokinin glucuronides for the selection of transgenic plant cells Okkels FT;Ward J;Joersbo M
  44. TIBTECH v.8 Engineering herbicide tolerance into crops Oxtoby E;Hughes MA
  45. Pestic Biochem Physiol v.62 The mode of action of isoxaflutole Ⅰ. Physiological effects, metabolism, and selectivity Pallett KE;Little JP;Sheekey M;Veerasekaran P
  46. Plant Physiol v.75 Site of action of chlorsulfuron Ray TB
  47. World J Microbiol Biotech v.10 Transgenic plants: performance, release and containment Sawahel WA
  48. Plant J v.19 Stable chloroplast transformation in potato: use of green fluorescent as a plastid marker Sidorov VA;Kasten D;Pang SZ;Hajdukiewicz PTJ;Staub JM;Nehra NS
  49. Plant Physiol v.121 Mannose induces and endonuclease responsible for DNA laddering in plant cell Stein JC;Hansen G
  50. Plant J v.22 A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency Sugita K;Kasahara T;Matsunaga E;Ebinuma H
  51. Plant Cell Rep v.18 Effective selection system for generating marker-free transgenic plants independent of sexual crossing Sugita K;Matsunaga E;Ebinuma H
  52. Planta v.208 Particle-bombarment-mediated co-transformation of elite Chinese rice cultivars with genes conferring resistance to bacterial blight and sap-sucking insect pests Tang KX;Tinjuangjun P;Xu Y;Sun XF;Gatehouse JA;Ronald PC;Qi HX;Lu XG;Christou P;Kohli A
  53. Guidance goes industry: use of antibiotic resistance marker genes in transgenic plants US Food;Drug Administration
  54. Biotech Adv v.13 Foreign gene delivery into monocotyledonous species Vain P;De Buyser J;Bui Trang V;Haicour R;Henry Y
  55. Plant Mol Biol v.5 A chimaeric hygromycin resistance gene as a selectable marker in plant cells Van Den Elzen PJM;Townsend J;Lee KY;Bedbrook JR
  56. Plant Mol Biol v.25 Molecular improvement of cereals Vasil IK
  57. Plant Mol Biol v.38 Cre/lox-mediated site-specific integration of Agrobacterium T-DNA in Arbidopsis thaliana by transient expression of cre Vergunst AC;Hooykaas PJJ
  58. Crit Rev Plant Sci v.18 Recombination in the plant genome and its application in biotechnology Vergunst AC;Hooykaas PJJ
  59. Nucl Acid Res v.26 Site-specific integration of Agrobacterium T-DNA in Arabidopsis thaliana mediated by Cre recombinase Vergunst AC;Jansen LET;Hooykaas PJJ
  60. Plant Cell Rep v.19 A mannose selection system for production of fertile transgenic maize plants from protoplast Wang AS;Evans RA;Altendorf PR;Hanten JA;Doyle MC;Rosichan JL
  61. Plant Mol Rep v.11 Selective agents and marker genes for use in transformation of monocotyledonous plants Wilmink A;Dons JJM
  62. Bio/Technology v.12 Transformation systems for generating marker-free transgenic plants Yoder JI;Goldbrough AP
  63. Nature Biotech v.18 Intrachromosomal recombination between attp as a tool to remove selectable marker genes from tobacco transgenes Zubko E;Scutt C;Meyer P