Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Expression of Chromium (VI) Reductase Gene of Heavy Metal Reducing Bacteria in Tobacco Plants

  • Jin, Tae-Eun (Dept. of Genetic Engineering, Hallym University) ;
  • Kim, Il-Gi (Dept. of Genetic Engineering, Hallym University) ;
  • Kim, Won-Sik (Dept. of Genetic Engineering, Hallym University) ;
  • Suh, Suk-Chul (NIAST in Rural Development Administration(RDA)) ;
  • Kim, Byung-Dong (Department of Horticulture, College of Agriculture and Life Science, Seoul National University) ;
  • Rhim, Seong-Lyul (Dept. of Genetic Engineering, Hallym University)
  • Published : 2001.01.01
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Abstract

A Chromium (VI)[Cr(VI)] reductase gene from heavy metal reducing bacteria Pseudomonas aeruginosa HP014 was used to transform tobacco plant cells. A chimeric construct containing the Cr(VI) reductase gene was transfered to tobacco leaf disks using an Agrobacteriun tumefaciens binary vector system. From the leaf disks, transformed plantlets were regenerated. Hybridization experiments demonstrated that the Cr(VI) reductase gene was inserted into and expressed in the regenerated plants. The Cr(VI) reduction activity showed that the transgenic plants may be a another possible tool to reduce the pollution of the toxic Cr(VI) in soil.

Keywords

References

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