Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of MS Medium Strength and Nitrogen Concentration on Bulblet Formation and Growth of Lilium In Vitro

MS 배지 내 무기물 농도 및 질소 농도가 나리의 자구 형성과 비대에 미치는 영향

  • 염미란 (충북대학교 첨단원예기술개발연구센터) ;
  • 박현춘 (충북대학교 첨단원예기술개발연구센터) ;
  • 양찬석 (충북대학교 첨단원예기술개발연구센터) ;
  • 백기엽 (충북대학교 첨단원예기술개발연구센터)
  • Published : 2001.11.01
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Abstract

To investigate the effects of MS medium strength and nitrogen concentration on bulblet formation from bulblet scale segment culture and bulblet growth from bulblet of Lilium oriental hybrid 'Casa Blanca', asiatic hybrid 'Mona', and longiflorum hybrid 'Hinomoto', 0.5∼2.0 strength of MS salts and 30∼120 mM nitrogen concentrations of MS medium were examined in vitro. The number of bulblets from bulb scale segment was favored in the strength of 0.5∼1.0 strength of MS salts or 30 mM total nitrogen concentration of MS medium in three cultivars. But the growth of bulblets formed in vitro was promoted in the 2 strength of MS medium or hight concentration of total nitrogen of MS medium up to 120 mM in three experimented cultivars.

본 실험에서 Lilium oriental hybrid 'Casa Blanca'. asiatic hybrid 'Mona'와 longiflorum hybrid 'Hinomoto'의 기내 인편으로부터의 자구 형성 및 비대를 위해 MS 배지 내 무기물 농도를 0.5∼2배로, 배지 내 총 질소 농도를 30∼120 mM로 달리하여 자구 형성과 생장을 조사한 결과 인편으로부터의 자구 형성수는 0.5와 1배의 MS 배지 무기물 농도 또는 30 mM의 총 질소 농도에서 'Mona' 품종이 다른 품종에 비해 가장 많았다. 'Casa Blanca'와 'Hinomoto'에서는 0.5배 MS 무기물 농도 또는 30 mM의 질소 농도가 자구 형성에 가장 효과적이었다. 자구 비대는 2배 MS 무기물 농도 또는 120 mM 총질소 농도에서 3품종 모두 양호하였다.

Keywords

References

  1. Acta Hort v.109 Effect of sucrose, mineral salts, and some organic substances on the adventitious regeneration in vitro of plantles from bulb-scale tissue of Lilium speciosum 'Rubrum' Aartrijk JV;Blom-Barnhoorn GJ
  2. Z. Pflanzenphysiol v.110 Adventitious bud formation from bulb scale explants of Lilium speciosum Thunb. in vitro. Effect of wounding, TIBA, and temperature Aartrijk JV;Blom-Barnhoorn GJ
  3. Potato Res v.28 Comparisons of cultivar performance on different nutrient media in a routine method for potato micropropagation Amiarouche L;Stuchbury T;Matthews S
  4. Plant Cell Tiss. Org. Cult v.49 Long term storage of lily: Effects of temperature and concentration of nutrients and sucrose Bonnier FJM;Van Tuyl JM
  5. J. Expt. Bot v.44 A preliminary study on the effects of nitrogen supply on the growth in vitro of 9 potato genotypes(Solanum spp.) Evnas NE
  6. Lilien. Ulmer. Stuttgart Feldmaier C;McRae J
  7. Plant culture media Commentary and Analysis George EF;Puttock DJM;George HJ
  8. Indian J. Exp. Biol v.16 Multiplication of Lilium longiflrum Thunb. by asceptic culture of bulb-scales and their segments Gupta P;Sharma AK;Chartuvedi HC
  9. J. Kor. Soc. Hort. Sci. v.26 Effects of inorganic salts in MS medium, sucrose, and activated charcoal on bulblet formation from in vitro bulb scales in Lilium oriental hybrid 'Casa Blanca' Han BH;Yae BW;Goo DH;Ko JY
  10. Plant Cell Tiss. Org. Cult v.37 Short-term studies of 15 $NO_3^-$and 15$NH_4^+$uptake by micropropagated strawberry shoots cultured with or without $CO_2$ enrichment Hdider C;Vezina LP;Desjardins Y
  11. Ann. Bot v.48 In vitro propagation og potato (Solanum tuberosum L.) Hussey G;Stacey NJ
  12. KGARR v.9 Production of virus-free stocks in oriental Lilies Kim JY;Han YH;Soh HS;Shim SW
  13. Kor. J. Plant Tess. Cult v.22 Regeneration of bulblets from of Lilium longiflorum Lee EM;Chung HJ;Lee YB
  14. J. Kor. Soc. Hort. Sci. v.39 Regeneration of bulblets from bulblet-derived bulb scales of Lilium longiflorum Lee EM;Chung HJ;Lee YB
  15. J. Plant Nutr. v.14 Wheat growth as affected by nitrogen type, pH and salinity;I. Biomass production and mineral composition Leidi EO;Silberbush M;Lips SH
  16. J. Kor. Soc. Hort. Sci. Hort v.17 Effect of light, inorganic salts and growth retardants on bulblet formation in Lilum Lem S;Seon JH;Son SH;Han BH;Paek KY
  17. Physiol. Plant v.15 A revised medium for rapid growth and bioassays with tobacco tissue cultures Murashige T;Skoog F
  18. Hort. Sci. v.14 Bulblet formation on Lilium longiflorum Thunb;'Nellie White'by foliar spray application of PBA Nightingale AE
  19. J. Kor. Soc. Hort. Sci. v.23 In vitro propagation of bulb scale sections of Lilium longiflorum Thunb Paek KY;Chun CK
  20. Hort. Res. Institute RDA Proceeding of symposium on the commercialization of lily production and breeding Park NB
  21. Sci. Hort v.5 Propagation of Lilium hybrids;I. Dependence of bulblet production on time of scale removal and growth substances Simmonds JA;Cumming BG
  22. J. Amer. Soc. Hort. Sci. v.107 Influence of agar concentration on in vitro shoot proliferation of Malus sp;'Almey'and Pyrus communis 'Seckel' Singha S
  23. Lilies of the world Woodcock HBD;Stearn WT