Virus Free Stock Production by In vitro Stem Cutting of Shoot Tip Cultures of Grapes

포도 경정배양에서 얻은 유묘의 기내삽목에 의한 무병묘 생산

  • Published : 2001.03.01

Abstract

The experiment was conducted to know the effect of plant growth regulators on axillary bud elongation from in vitro stem cutting and the possibility of virus-free stock production. Axillary buds were well elongated in 3/4 strength MS medium supplemented with 0.1 or 0.5 mg/1 BA and 0.05 mg/1 NAA. Transferred plantlets could be established well in vermiculite and peat moss mixture (3:1, v/v) compare to other mixtures. In virus indexing, all the varieties of mother plants were infected by GLRV Ⅲ. Infected percentages of the three varieties were ranged from 30% to 75%. But negative response was revealed against the other species of virus, GLRV Ⅰ, GFLV and ArMV. Plantlet of 'Schuyler' and 'Muscat of Alexandria', which were cultured in vitro, showed positive response against GLRV Ⅲ and infected percentage of the former was 37.5% but the latter, 12.5%. On the other hand, that of 'Campbell Early' negativiely responded against all the species of virus indexed.

포도의 기내 삽목방법을 이용하였을 때 액아의 신장에 미치는 생장조절제의 영향과 이들 유묘의 virus 이병 정도에 관해 검정한 결과를 요약하면 다음과 같다. 3/4 MS 기본배지에 BA 0.1 또는 0.5 mg/L와 NAA 0.05 mg/L를 혼용한 것이 액아로부터 신초의 신장에 적합하였다. 기외이식용 상토는 vermiculite와 peat moss를 3: 1 등량용적 비율로 혼합한 경우 활착률이 가장 높았다. 포장에서 재배되고 있던 'Schuyler'외 2품종을 ELISA 검정한 결과 3품종 모두 GLRV Ⅰ, GFLV, ArMV에는 음성반응을 나타내었으나 GLRV Ⅲ에는 감염되 어 있었으며, 이병률은 30∼75%로 품종 간 차이가 현저하였다. 경정배양에서 얻은 유묘의 virus 검정 결과, GLRV Ⅰ, GFLV, ArMV는 3품종 모두 음성반응을 나타내었으나 'Schuyler'와 'Muscat of Alexandria'는 GLRV Ⅲ에 양성반응을 나타내어 이병률은 각각 37.5 %와 12.5%였다.

Keywords

References

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