Biomedical Science Letters (대한의생명과학회지)
- Volume 7 Issue 2
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- Pages.85-89
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- 2001
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
Detection of Enterotoxins in Staphylococcus aureus Isolated from Clinical Specimens and Kimbap Using Multiplex PCR
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Kim, Jong-Bae
(Department of Biomedical Laboratory Sciences, College of Health Sciences, Yonsei University) ;
- Kim, Hong (Department of Biomedical Laboratory Sciences, College of Health Sciences, Yonsei University) ;
- Jin, Hyun-Seok (Department of Biomedical Laboratory Sciences, College of Health Sciences, Yonsei University) ;
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Kim, Young-Sam
(Department of Biomedical Laboratory Sciences, College of Health Sciences, Yonsei University) ;
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Kim, Keun-Sung
(Department of Food Science and Technology, College of Industrial Sciences, Chung-Ang University) ;
- Kang, Yun-Sook (Department of Food Evaluation, Korea Food and Drug Administration) ;
- Park, Jong-Seok (Department of Food Evaluation, Korea Food and Drug Administration) ;
- Lee, Dong-Ha (Department of Food Evaluation, Korea Food and Drug Administration) ;
- Woo, Gun-Jon (Department of Food Evaluation, Korea Food and Drug Administration)
- Published : 2001.06.01
Abstract
Many Staphylococcus aureus strains produce enterotoxins causing food poisoning. Staphylococcal enterotoxins are classified by serological criteria into five major groups - subtype A to E. It is difficult, time-consuming, and expensive to detect staphylococcal enterotoxins in the clinical laboratory. In this study, we fried to detect the enterotoxin genes of Staphylococcus aureus strains isolated from clinical specimens and Kimbap - rice rolled in a sheet of laver - using multiplex PCR technique. A total of 77 strains of Staphylococcus aureus from clinical specimens and 78 strains from Kimbap were isolated. Among clinical isolates of S. aureus, 60 strains (78.0%) were identified as producing enterotoxins. A total offs strains (91.6%) in the 60 staphylococcal enterotoxin producing strains were enterotoxin subtype C. In case of kimbap: 43 (55.1%) strains were detected to produce enterotoxins and 39 (90.6%) enterotoxin producing strains were subtype A.