Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Detection of Enterotoxins in Staphylococcus aureus Isolated from Clinical Specimens and Kimbap Using Multiplex PCR

  • Kim, Jong-Bae (Department of Biomedical Laboratory Sciences, College of Health Sciences, Yonsei University) ;
  • Kim, Hong (Department of Biomedical Laboratory Sciences, College of Health Sciences, Yonsei University) ;
  • Jin, Hyun-Seok (Department of Biomedical Laboratory Sciences, College of Health Sciences, Yonsei University) ;
  • Kim, Young-Sam (Department of Biomedical Laboratory Sciences, College of Health Sciences, Yonsei University) ;
  • Kim, Keun-Sung (Department of Food Science and Technology, College of Industrial Sciences, Chung-Ang University) ;
  • Kang, Yun-Sook (Department of Food Evaluation, Korea Food and Drug Administration) ;
  • Park, Jong-Seok (Department of Food Evaluation, Korea Food and Drug Administration) ;
  • Lee, Dong-Ha (Department of Food Evaluation, Korea Food and Drug Administration) ;
  • Woo, Gun-Jon (Department of Food Evaluation, Korea Food and Drug Administration)
  • Published : 2001.06.01
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Abstract

Many Staphylococcus aureus strains produce enterotoxins causing food poisoning. Staphylococcal enterotoxins are classified by serological criteria into five major groups - subtype A to E. It is difficult, time-consuming, and expensive to detect staphylococcal enterotoxins in the clinical laboratory. In this study, we fried to detect the enterotoxin genes of Staphylococcus aureus strains isolated from clinical specimens and Kimbap - rice rolled in a sheet of laver - using multiplex PCR technique. A total of 77 strains of Staphylococcus aureus from clinical specimens and 78 strains from Kimbap were isolated. Among clinical isolates of S. aureus, 60 strains (78.0%) were identified as producing enterotoxins. A total offs strains (91.6%) in the 60 staphylococcal enterotoxin producing strains were enterotoxin subtype C. In case of kimbap: 43 (55.1%) strains were detected to produce enterotoxins and 39 (90.6%) enterotoxin producing strains were subtype A.

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