Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Differential Expression of Spin Transcripts: Oocyte and Somatic Types

  • Oh, Bermseok (National Institute of Health, Division of Genetic Disease) ;
  • Hwang, Sue-Yun (Institute of Immunobiology, Catholic Institutes of Medical Science, The Catholic University of Korea) ;
  • Solter, Davor (Max-Planck Institute of Immunology) ;
  • Knowles, Barbara (The Jackson Laboratory)
  • Published : 2001.03.01
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Abstract

Spin is an abundant maternal transcript comprising up to 0.2% of the total mRNA stock in mouse oocyte, whose protein product is associated with the meiotic spindle. We have identified a new isoform of Spin transcript containing a distinct 5'-untranslated region and the N-terminus of encoded protein. Northern blot and RT-PCR analysis showed that the new isoform is expressed in embryos and most of adult tissues, while the previously identified transcript is expressed solely in mouse oocyte. We thus designated these two Spin isoforms as somatic type and oocyte type, respectively. To investigate the underlying mechanism for the differential expression, genomic structure of Spin was examined. Spin exists as multiple copies in the genome, some of which appears to be pseudogenes, and characterization of Spin genomic clones indicates that oocyte- and somatic-isoforms were generated by alternative splicing. The complex organization of Spin genomic locus and its multifaceted control of expression provide a good model to study the molecular mechanisms of elaborate genome usage in mammals.

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References

  1. Huarte J, Belin D, Vassalli, A, Stricklaud S, aud Vassalli J-D (1987) Meiotic maturation of mouse oocytes triggers the translation and polyadenylation of dormant tissue-type plasminogen activator mRNA. Genes & Dev 1: 1201-1211 https://doi.org/10.1101/gad.1.10.1201
  2. Oh B, Hampl A, Eppig JJ, S olter D, and Knowles BB (1998) SPIN, a substrate in the MAP kinase pathway in mouse oocytes. Mol Rep Dev 50: 240-249 https://doi.org/10.1002/(SICI)1098-2795(199806)50:2<240::AID-MRD15>3.0.CO;2-A
  3. Oh B, Hwang S -Y, McLaughlin J, S olter D, and Knowles BB (2000) Timely translation during the mouse oocyte-to-embryo transition. Development 127: 3795-3803
  4. Oh B, Hwang S-Y, Solter D, and Knowles BB (1997) Spindlin, a major maternal transcript expressed in the mouse during the transition from oocyte to embryo. Development 124: 493-503
  5. Richter J (1999) Cytoplasmic polyadenylation in development and beyond. Microbiol Mol Biol Rev 1999:446-456
  6. Rothstein J, Johnson D, DeLoia JA, Skowronski J, Solter D, and Knowles BB (1992) Gene expression during preimplantation mouse development. Genes & Dev 6: 1190-1201 https://doi.org/10.1101/gad.6.7.1190
  7. Sambrook J, Fritsch E, and Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
  8. Vriz S, Taylor M, and Mechali M (1989) Differential expression of two Xenopus c-myc proto-oncogenes during development. EMBO J 8: 4091-4097
  9. Wolffe A and Brown DD (1988) Developmental regulation of two 5S ribosomal RNA genes. Science 241: 1626-1632 https://doi.org/10.1126/science.3420414