Research in Plant Disease (식물병연구)
- Volume 7 Issue 3
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- Pages.164-169
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- 2001
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- 2233-9191(eISSN)
Identification and Characterization of Tobamoviruses Isolated from Commercial Pepper Seeds
시판 고추 종자에서 분리한 Tobamovirus의 동정 및 특성 조사
Abstract
Two Tobamoviruses showing different local lesion types on Nicotiana glutinosa was isolated from commercial pepper seeds. These viruses were designated Tobamovirus-6 (T-6) and Tobamovirus-19 (T-19). The biological and serological assays revealed that T-6 and T-19 were closely related to Pepper mild mottle virus (PMMoV) and Tomato mosaic virus (ToMV), respectively, The isolates also had low similarity in the array of viral coat protein gene sequences, of which T-19 was most identical to known strains of ToMV, while T-6 was closely related to PMMoV.
Nicotiana glutinosa를 이용한 단병반분리를 통해 국내에 시판되고 있는 상용고추종자로부터 괴사국부병반의 크기가 다른 두 종류의 Tobamovirus를 분리하고 이들을 각각 Tobamovirus-6(T-6)과 Tobamovirus-19(T-19)로 명명하였다. 기주범위와 혈청학적 연관성 조사에서 T-6은 pepper mild mottle vitus(PMMoV)와, T-19는 tomato mosaic virus (ToMV)와 유사한 특성을 보였다. 또한 외피단백질 염기서열 분석에서 T-6은 PMMoV와 T-19는 ToMV와 매우 유사하였다.