YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Identification and Characterization of a Novel Cytosolic Phospholipase from Bovine Red Blood Cells

소 적혈구로부터 새로운 형태의 세포질 포스포리파제 의 동정 및 특성규명

  • 신혜숙 (중앙대학교 약학대학 위생약학 교실) ;
  • 김하동 (중앙대학교 약학대학 위생약학 교실) ;
  • 장동훈 (중앙대학교 약학대학 위생약학 교실) ;
  • 전형준 (중앙대학교 약학대학 위생약학 교실) ;
  • 유충규 (이화여자대학교 약학대학 약품분석화학 교실)
  • Published : 2001.08.01
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Abstract

A $Ca^{2+}$_dependent PLA$_2$activity termed rPLA$_2$, was detected in the cytosol of bovine red blood cells (RBCs). The rPLA$_2$was characterized as a similar form to Group IV cPLA$_2$, but different in several column chromatographic profiles including an anion exchange column. To examine whether this rPLA$_2$is different from the well characterized cPLA$_2$, a quinone derivative, BJ50, was developed and tested for the inhibitory effect on the two PLA$_2$enzymes. The rPLA$_2$activity was inhibited by a quinone derivative (BJ50) with ID$_{50}$ of 20 $\mu$M., but had no effect on the cPLA$_2$, suggesting that rPLA$_2$may be a novel type of cytosolic PLA$_2$in RBCs.s.s.

Keywords

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