The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Use of Gelatin Particle Agglutination Test for the Detection of Cymbidium mosaic virus in Cattleya Plants

  • Han, Jung-Heon (Division of Applied Biology and Chemistry, Seoul National University) ;
  • Jeong, Hyoo-Won (Division of Applied Biology and Chemistry, Seoul National University) ;
  • La, Yong-Joon (Division of Applied Biology and Chemistry, Seoul National University)
  • Published : 2001.12.01
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Abstract

Gelatin particle agglutination test (GPAT) was used to detect Cymbidum mosaic virus (CymMV) in Cattleya plants. Gelatin particles were coated with purified anti-CymMV immunoglobulin of 25-100 $\mu\textrm{g}$/ml and were subjected to several different concentrations of purified CyMfV as well as varying dilutions of orchid leaf extracts. The GPAT detected purified CymMV up to a minimum concentration of 10 $\mu\textrm{g}$/ml. CymMV was detected from crude sap extract of infected Cattleya leaves and roots up to 1:51,200 and 1:25,600 dilutions, respectively. However, the optimum range of leaf and root sap dilutions was between 50-100. Non-specific reactions were not encountered from any of the healthy orchid plants tested. The entire GPAT process was completed within 2-3 hours. This test was found to be very useful for the detection of CymMV in orchids because it is sensitive, economical, and easy to perform.

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