YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effect of the Water Extract of Ajbizzia julibrissin on Apoptotic Cell Death in the Human Leukemic Jurkat T Cell Line

백혈병세포주 Jurkat T 세포에서 합환피 (Ajbizzia julibrissin) 물 추출물의 아포토시스 유도 효과

  • 황상구 (원광대학교 한의과대학 한의학과 병리학교실) ;
  • 이형철 (원광대학교 한의과대학 한의학과 병리학교실) ;
  • 김춘관 (원광대학교 한의과대학 한의학과 병리학교실) ;
  • 김용익 (원광대학교 한의과대학 한의학과 병리학교실) ;
  • 주성민 (원광대학교 자연과학대학 생명과학부) ;
  • 김원신 (원광대학교 자연과학대학 생명과학부) ;
  • 전병훈 (원광대학교 자연과학대학 생명과학부)
  • Published : 2001.12.01
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Abstract

Apoptosis is a morphologically and biochemically distinct form of cell death that occurs in many different cell types in a wide variety of organisms. Ajbizzia julibrissin belonging the family Leguminosae has been used for the treatment of contusion, sore throat, amnesia, and insomnia in oriental traditional medicine. This study investigates whether the water extract off julibrissin induce apoptotic cell death in Jurkat T-acute lymphoblastic leukemia (ALL) cells. Jurkat cells were increased inhibitions of cell viability in a concentration-dependent manner by A julibrissin. This herbal medicine also caused apoptosis as measured by cell morphology and DNA fragmentation. The capability oft julibrissin to induce apoptosis was associated with proteolytic cleavage of specific target protein such as poly (ADP-ribose) polymerase (PARP) protein suggesting the possible involvement of caspases. Our result skewed that Bcl-2 and Bax protein levels were not changed in all A julibrissin-treated groups compared to control group. These results suggest that A julibrissin-mediated apoptosis is independent with Bcl-2 related signaling pathway in this cells.

Keywords

References

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