약학회지 (YAKHAK HOEJI)

  • 제45권6호
  • /
  • Pages.724-729
  • /
  • 2001
  • /
  • 0377-9556(pISSN)
  • /
  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

느릅나무의 에틸 아세테이트 추출물에 의한 Melanin생성 효과

Effects of Ethyl Acetate Extract from Ulmus davidiana var. japonica on Melanogenesis

  • 천현자 (원광대학교 한의학 전문대대학원) ;
  • 정승일 (원광대학교 한의학 전문대대학원) ;
  • 김일광 (원광대학교 자연과학대학 화학과)
  • 발행 : 2001.12.01
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초록

Melanogenesis is a physiological process resulted in the synthesis of melanin pigments, which has a role in protecting skin front the damaging effect of ultra-violet (UV) radiation. The main aim of the present study was to examine the effect of Ulmus davidiana var. japonica(UL) on Melanogenesis. Cells were cultured in the presence of various concentrations of Ulmus davidiana var. japonica for 48 h, and there were estimated total melanin contents as a final product and activity of tyrosinase, a key enzyme, in Melanogenesis. Among the four solvent extracts tested, EtOAc extract mostly increased tyrosinase activity, And EtOAc extract increased the melanin contents and tyrosinase activity in a dose-dependent manner. Especially It was observed that 100$\mu\textrm{g}$/ml EtOAc extract promotes melanin secretion in B16/F10 melanoma cells by 140% at 48 h treatment and activity of tyrosinase increased by 180% in the presence of same concentration. In conclusion, as for EtOAc extract treatment, there was no effect on the viability of B16/F10 cell, only to stimulate Melanogenesis.

키워드

참고문헌

  1. 대한식물도감 이창복
  2. 한국약품식물자료도감 육창수
  3. 약품식물학각론 약품식물학연구회
  4. 동의보감 동의학연구소
  5. 중약대사전 김창민(외)
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