YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Antitumor Immunomodulatory Activity of PVP a Protein-polysaccharide Fraction Prepared from a Wild Mushroom Psathyrella velutina

큰눈물버섯 (Psathyrella velutina)으로부터 분리한 단백다당체 PVP의 항암 . 면역활성

  • 정경수 (충남대학교 약학대학 미생물면역학교실) ;
  • 이지선 (충남대학교 약학대학 미생물면역학교실)
  • Published : 2001.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

A protein-polysaccharide fraction of a Korean wild mushroom Psathyrella velutina, PVP, was prepared and its antitumor immunomodulatory activity was investigated. When PVP was administered once daily for seven days from day 1 to day 7 into male ICR mice implanted with 1 $\times$ 10$^{5}$ cells of sarcom 180 tumor cells into the peritoneum on day 4, it inhibited the growth of sarcoma 180 cells by 92.8%. In XTT assay, PVP also exhorted in vitro anti-proliferation activity on U-937, a human monoblastoid cell line, as well as sarcoma 180 cells. PVP showed marked stimulatory activity on the immune system in that it induced the accumulation of PEC (the stimulation index, Sl=4.90 at 100 mg/kg), stimulated the BALB/c mouse splenic lymphocytes to form lymphoblasts (Sl=5.75 at 100$\mu\textrm{g}$/ml), and upergulated the expression of CD25 molecules. All these results strongly support that PVP exhorts its antitumor activity through stimulation of the immune system as well as anti-proliferative activity on the tumor cells.

Keywords

References

  1. Yakhak Hoeji v.31 no.126 Antitumor Activity of the Polysaccharide-Fraction (Copolang) from Coriolus versicolor and its Effects on the Immue Function Moon, C.-K.;Lee, S.-H.;Mock, M.-S.;Kim, D.-O.
  2. J. Kor. Cancer Assoc. v.19 no.95 Effects of Copolang on Murine Immune Function and Antitumor Activity Jo, S.-K.;Kim, S.-H.;Yoon,T.-K.
  3. Yakhak Hoeji v.38 no.158 Antitumor activity of Kp, a protein-polysaccharide from the mycelial culture of Phellinus linteus Chung, K.S.;Kim, S.S.;Kim, H.S.;Han, M.W.;Kim, B.K.
  4. Arch. Pharm. Res. v.16 no.336 Effect of Kp, an antitumor protein-polysaccharide from mycelial culture of Phellinus linteus on the humoral immune response of tumor-bearing ICR mice to sheep red blood cells Chung, K.S.;Kim, S.S.;Kim, H.S.;Kim, K.Y.;Han, M.W.;Kim, B.K.
  5. Cancer Res. v.30 no.2776 Fraction of the especially lentinan, from Lentinus edodes (an edible mushroom) Chihara, G.;Hamuro, J.;Maeda, Y.Y.;Arai, Y.;Fukuoka, F.
  6. Yakhak Hoeji v.41 no.105 Immunoactivities of the protein-polysaccharides of the tips of the growing carpophores of Ganoderma lucidum Chung, K.S.;Kim, S.B.;Chung, S.H.
  7. Yakhak Hoeji v.42 no.487 Flow cytometrical analysis of the antitumor and immunomodulatory activities of GLB-A and GLB-B, the protein-polysa-ccharide fractions of the growing tips of Ganoderma lucidum Oh, J.Y.;Chung, K.S.
  8. Yakhak Hoeji v.44 no.463 Antitumor and immunomodulatory activity of Lycoperdon pedicellatum Chung, K.S.;Kim, J.H.
  9. Yakhak Hoeji v.45 no.64 Flow cytometrical investigation on antitumor activity of mycelial culture of insect-born fungus Paecilomyces japonica DGUM 32001 Lee, J.S.;Lee, I.S.;Chung, K.S.;Kim, Y.H.;Han, Y.H.;Lee, M.H.
  10. The Journal of Biological Chemistry v.264 no.173 Purification and properties of an N-acetylglucosamine-specific lectin from Psathyrella velutina mushroom Kochibe, N.;Matta, K.L.
  11. J. Biol. Chemistry v.267 no.707 Carbohydrate binding specificity of immobilized Psathyrella velutina lectin Endo, T.;Ohbayashi, H.;Kanazawa, K.;Kochilbe, N.;Kobata, A.
  12. J. Biochem. v.126 no.530 Multi-specificity of a Psathyrella velutina mushroom lectin: heparin/pectin binding occurs at a site uraminic acid-specific site Ueda, H.;Saitoh, T.;Kojima, K.;Ogawa, H.
  13. Cancer Res. v.48 no.4827 Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines Scudiero, D.A.;Shoemaker, R.H.;Paull, K.D.;Monks, A.;Tierney, S.;Nofziger, T.H.;Currens, M.J.;Seniff, D.;Boyd, M.R.
  14. J. Natl. Cancer Inst. v.81 no.577 New soluble-formazan assay for HIV-1 cytopathic effects: application to high-flux screening of synthetic and natural products for AIDS-antiviral activity Weislow, O.S.;Kiser, R.;Fine, D.L.;Bader, J.;Shoemaker, R.H.;Boyd, M.R.
  15. J. Immunol. Methods v.142 no.257 An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT. Roehm, N.W.;Rodgers, G.H.;Hatfield, S.M.;Glasebrook, A.L.
  16. Methods in microbiology v.5B Norris, J.R.;Ribbons, D.W.
  17. Anal. Biochem. v.116 no.53 Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein Read, S.M.;Northcote, D.H.