Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Production of Transgenic Chicken by Using Embryo Culture Techniques

수정란 배양 기술을 이용한 형질전환 닭 생산

  • I. S. Jeon (National Livestock Research Institute, RDA) ;
  • D. H. Yoon (National Livestock Research Institute, RDA) ;
  • E. W. Park (National Livestock Research Institute, RDA) ;
  • W. G. Nho (National Livestock Research Institute, RDA) ;
  • C. H. Choi (National Livestock Research Institute, RDA) ;
  • J. H. Lee (National Livestock Research Institute, RDA) ;
  • H. H. Lee (National Livestock Research Institute, RDA) ;
  • I. C. Cheong (National Livestock Research Institute, RDA) ;
  • J. Y. Han (School of Agricultural Biotechnology, Seoul National University)
  • Published : 2001.08.01
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Abstract

The goal of this paper was to examine the qualify zygote-acquiring method for in-vitro culture and the in-vitro culture method of the acquired zygote from a technological perspective. We have reported the results on the introduction of foreign DNAs using the described culturing method. After performing in-vitro and surrogate eggshell culture on a zygote acquired from the abdomen of a hen, 25.8% hatchability was acquired. After microinjecting foreign DNAs into the acquired zygote and performing in-vitro and surrogate eggshell culture using the same method, 13.1∼11.7% hatchability was acquired. Having compared the developments of the control subjects and the experimental subjects, the viability of the experimental subjects on the 4∼5th day of culturing was much lower compared to that of the control subjects. This is a result that shows that the microinjection process of foreign DNAs might have a negative effect on the existence of the embryo; therefore, various technical attempts should be made to minimize such negative effects. Having microinjected foreign DNAs into the zygote of a hen to produce transgenic chickens, 3 transgenic founders were Produced and 70 G1 progeny were produced as a result of the progeny test that had been performed to the present.

Keywords

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