Korean Journal of Veterinary Pathology (한국수의병리학회지)
- Volume 5 Issue 1
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- Pages.29-34
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- 2001
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- 1226-766X(pISSN)
Chemopreventive Effect of Chitosan on Rat Colon Carcinogenesis Induced by Azoxymethane
실험적 대장암 모델에서 키토산의 발암 억제효과에 관한 연구
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Han, Beom-Seok
(General Toxicology Department, National Institute of Toxicological Research, KFDA) ;
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Kim, Dae-Joong
(College of Veterinary Medicine, Chungbuk University) ;
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Ahn, Byeong-Woo
(General Toxicology Department, National Institute of Toxicological Research, KFDA) ;
- Kim, Ki-Sok (General Toxicology Department, National Institute of Toxicological Research, KFDA) ;
- Kang, Jin-Seok (General Toxicology Department, National Institute of Toxicological Research, KFDA) ;
- Moon, Ji-Young (General Toxicology Department, National Institute of Toxicological Research, KFDA) ;
- Hong, Choong-Man (General Toxicology Department, National Institute of Toxicological Research, KFDA) ;
- Jang, Dong-Deuk (General Toxicology Department, National Institute of Toxicological Research, KFDA)
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한범석
(국립독성연구소 일반독성부) ;
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김대중
(충북대학교 수의과대학) ;
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안병우
(국립독성연구소 일반독성부) ;
- 김기석 (국립독성연구소 일반독성부) ;
- 강진석 (국립독성연구소 일반독성부) ;
- 문지영 (국립독성연구소 일반독성부) ;
- 홍충만 (국립독성연구소 일반독성부) ;
- 장동덕 (국립독성연구소 일반독성부)
- Published : 2001.06.01
Abstract
This study was conducted to assess the chemopreventive effects of chitosan in a rat colon carcinogenesis induced by azoxymethane (AOM). Ninety, 5-week-old, male F344 rats were divided into three groups. The animals in group 1 received subcutaneous injections of 15mg/kg AOM three times for two weeks, then were placed on powdered basal diet containing 2% chitosan for 37 weeks from weeks 3 to 40. The animals in group 2 were given AOM alone. The animals in group 3 were given 2% chitosan without prior carcinogen treatment. All animals were sacrificed at week 12 for quantitative analysis of aberrant crypt foci (ACF) and at week 40 fur analysis of tumor induction. Total numbers of ACF and AC per colon of group 1 were not significantly different from those of group 2. Tumor incidences and multiplicities of small intestine in the group 1 were significantly decreased compared with those of the group 2 (P<0.05). According to pathological diagnoses, adenocarcinoma incidence and multiplicity in the small and large intestine in the group 1 were significantly decreased compared with those of the group 2 (p<0.05). No toxic effects were observed in animals given chitosan in terms of body weights, and liver or kidney histology. These results indicate that chitosan may have a potential as chemopreventive agents of colon carcinogenesis during the postinitiation stage.