Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Preservation of Marine Heterotrophic Bacteria by Using a Deep-freezing Method

  • Park, Shin-Hye (Microbiology Laboratory, Korea Ocean Research & Development Institute) ;
  • Lee, Hyun-Sang (Microbiology Laboratory, Korea Ocean Research & Development Institute) ;
  • Lee, Hong-Kum (Microbiology Laboratory, Korea Ocean Research & Development Institute)
  • Published : 2001.09.01
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Abstract

The effect of cryoprotectants and suspending solutions on the preservation of marine heterotophic bacteria was investigated. Six halotolerant and four halophilic bacterial isolates suspended in either distilled water or artificial seawater were preserved in glycerol and dimethylsulfoxide at -70$\^{C}$, respectively. After one year of preservation, the recovery rates on the appropriate agar plates were estimated. The survival rate was found to be dependent on the strain tested, regardless of the preservation conditions tested.

Keywords

References

  1. Adv.Appl.Microbiol. v.43 Marine microorganisms as a source of new natural products Beran V.S.;M.Greenstein;W.M.Maise
  2. FEMS Microbiol.Lett. v.84 Antibiotic production by the marine photosynthetic bacterium Chromatium pupuratum NKPB 031704: localization of activity to the chromatophores Burgess J.G;H.Miyashita;H.Sudo;T.Matsunaga
  3. Annu.Rev.Microbiol. v.48 Strategies for the discovery of secondary metabolites from marine bacteria: ecological perspectives Fenical W.;P.R.Jensen
  4. J.Appl.Bacteriol. v.33 Further studies on long term preservation of marine bacteria Greig M.A;M.S.Hendrie;J.M.Shewan
  5. Adv.Appl.Microbiol. v.24 Preservation of microorganisms Heckly R.J.
  6. Eur.J.Biochem. v.123 An extracellular polysaccharide produced by Zoogloea ramigera 115 Ikeda F.;H.Shuto;T.Fukui;K.Tomita
  7. J.Antibiot. v.50 New anticancer antibiotics pelatiomicins,produced by a new marine bacterium Peiobacter variablis Imamura N.;M.Nishijima;T.Takadera;K.Adach
  8. Microbial Culture Isaac S.;D.Jennings
  9. Maintenance of microorganisms Kirsop B.E.;J.J.S.Snell
  10. Chem.Rev. v.93 Bioactive metabolites of symbiotic marine microorganisms Kobayashi J.;M.Ishibashi
  11. Int.J.Syst.Evol.Microbiol. v.51 Hahella chejuensis gen.nov.sp.nov.an extracellular-polysaccharide producing marine bacterium Lee H.K;J.Chun;E.Y.Moon;S.H.Ko;D.S.Lee;H.S.Lee;K.S.Bae
  12. J.Microbiol. v.37 Bacterial diversity of culturable isolates from seawater and a marine coral.Plexauridae sp.,near Mun-Sum,Cheju-Island Lee J.H;H.H.Shin;D.S.Lee;K.K.Kwon;S.J.Kim;H.K.Lee
  13. Caryobiology v.14 The role of intracellular freezing in the death of cells cooled at supraoptimal rates Mazur P.
  14. Abstr.2nd Asia-Pacific Marine Biotechnology Conference and 3rd Asia-Pacific Conference of Algal Biotechnology Cryopreservation of four species of marine phytoplankton using different type and concentration of cryoprotectants Omar H.;I.Ishak
  15. J.Biotech. v.70 The bioporcess-technological potential of the sea Pompeni S.A.
  16. Appl.Microbiol. v.30 Survival of frozen mycoplasmas Racchach M.;S.Rottem;S.Razin
  17. Appl.Environ.Microbiol. v.62 Description of a new polymer-secreting bacterium from a deep-sea hydrothermal vent,Alteromonas macleodii subsp.fijiensis,and preliminary characterization of the polymer Rafuences G.;P.Pignet;A.Peres;R.Christen;H.Rougeaux;G.Barbier;J.Guezennec
  18. Maintenance of microorganisms General introduction to maintenance methods Snell J.J.S.;Kirsop B.E.(ed.);J.J.S.Snell(ed.)
  19. J.Biotech. v.70 Biodiversity and potentials of marine-derived microorganisms Sponga F.;L.Cavaletti;A.Lazzarini;A.Borghi;I.Ciciliato;D.Losi;F.Marinelli