Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Stress-shock Response of a Methylotrophic Bacterium Methylovorus sp. strain SSl DSM 11726

  • Park, Jong H. (Department of Biology and Institute of Life Science and Biotechnology, Yonsei University) ;
  • Kim, Si W. (Department of Environmental Engineering, College of Engineering, Chosun University) ;
  • Kim, Eungbin (Department of Biology and Institute of Life Science and Biotechnology, Yonsei University) ;
  • Young T. Ro (Laboratory of Biochemistry, College of Medicine, Konkuk University) ;
  • Kim, Young M. (Department of Biology and Institute of Life Science and Biotechnology, Yonsei University)
  • Published : 2001.09.01
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Abstract

Methylovorus sp. strain SS1 DSM 11726 was found to grow continuously when it was transferred from 30$\^{C}$ to 40$\^{C}$ and 43$\^{C}$. A shift in growth temperature from 30$\^{C}$ to 45$\^{C}$, 47$\^{C}$ and 50$\^{C}$ reduced the viability of the cell population by more than 10$^2$, 10$^3$and 10$\^$5/ folds, respectively, after 1h cultivation. Cells transferred to 47$\^{C}$ and 50$\^{C}$ after preincubation for 15 min at 43$\^{C}$, however, exhibited 10-fold increase in viability. It was found that incubation for 15 min at 40$\^{C}$ of Methylovorus sp. strain SSl grown at 30$\^{C}$ was sufficient to accelerate the synthesis of a specific subset of proteins. The major heat shock proteins had apparent molecular masses of 90, 70, 66, 60, and 58 kDA. The 60 and 58 kDa proteins were found to cross-react with the antiserum raised against GroEL protein. The heat shock response persisted for over 1h. The shock proteins were stable for 90 min in the cell. Exposure of the cells to methanol induced proteins identical to the heat shock proteins. Addition of ethanol induced a unique protein with a molecular mass of about 40 kDa in addition to the heat-induced proteins. The proteins induced in paraquat-treated cells were different from the heat shock proteins, except the 70 and 60 kDa proteins.

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