Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Anti-tumor Activity of the Fruitbody Extract of Basidiomycete, Phellinus linteus

  • Lim, Jong-Soon (College of Oriental Medicine Institute of traditional Medicine and Bioscience, Daejeon University) ;
  • Kim, Seung-Hyung (College of Oriental Medicine Institute of traditional Medicine and Bioscience, Daejeon University) ;
  • Park, Jin-Seo (Department of Microbiology, College of Sciences Institute of traditional Medicine and Bioscience, Daejeon University) ;
  • Park, Jeong-Youl (Department of Microbiology, College of Sciences Institute of traditional Medicine and Bioscience, Daejeon University) ;
  • Park, Seong-Joo (Department of Microbiology, College of Sciences Institute of traditional Medicine and Bioscience, Daejeon University) ;
  • Shin, Kwang-Soo (Department of Microbiology, College of Sciences Institute of traditional Medicine and Bioscience, Daejeon University)
  • Published : 2001.06.01
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Abstract

Methanol extract prepared from the fruitbody of Phellinus linteus (EPL) showed anti-tumor and immune-stimulating activities. The invasion activity of Bl6-F10 melanoma cells through a reconstituted basement membrane to the collagen-coated lower surface of the filters was inhibited about 67% by EPL (100 $\mu\textrm{g}$/ml). Also, EPL inhibited the expression of the mRNA for MMP-2 and MMP-9. In vivo treatment of C57BL/6 mice (150 mg/kg) with EPL for 14 days, the pulmonary colonization was found to be inhibited about 75%. Using reverse transcriptionpolymerase chain reaction (RT-PCR) analysis, we found that cytokine IL-12 and INF-${\gamma}$ genes were induced by EPL. Furthermore, EPL stimulated the proliferation of CD4$\^$+/(33.5%) and CD8$\^$+/(17.7%) in mouse splenocytes.

Keywords

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