Journal of Microbiology

  • 제39권1호
  • /
  • Pages.42-48
  • /
  • 2001
  • /
  • 1225-8873(pISSN)
  • /
  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Regulation of Actin Gene Expression During the Differentiation of Naegleria gruberi

  • 발행 : 2001.03.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The regulation of actin gene expression during the differentiation of Naegleria gruberi was examined. Actin mRNA concentration was maximal in amoebae and decreased rapidly after the initiation of differentiation. At 20 min after initiation, the concentration of actin mRNA decreased to 55% of the maximal value. The actin mRNA concentration decreased to the minimum at 80 min (15% of the maximum), and then began to increase slightly at the end of differentiation. This decrease of actin mRNA concentration was regulated by the repression of actin gene transcription based on nuclear run-on transcription experiments. The rates of transcription of actin gene in nuclei prepared at 40 and 80 min after the initiation of differentiation were 50 and 28% of that of nuclei prepared at the beginning of differentiation, respectively. The addition of cycloheximide at the initiation of differentiation inhibited both the rapid decrease in the concentration of actin mRNA and the repression of actin gene transcription. These results suggest that the rapid decrease in the concentration of actin mRNA during the differentiation of N. gruberi is accomplished by the repression of actin gene transcription and this transcriptional regulation requires continuous protein synthesis during the differentiation.

키워드

참고문헌

  1. Exp. Cell. Res. v.219 Independent mechanisms are utilized for the coordinate and transient accumulation of two differentiation specific mRNAs during differentiation of Naegleria gruberi amoebae into flagellates Bok J.W.;Y.J. Jin;J.H. Lee
  2. Dissertation for Master Thesis Study of α-tubulin gene promoter of Negleria gruberi Cheon H.J.
  3. J. Microbiol. v.33 Cloning and sequence determination of α-tubulin, β-tubulin and flagellar calmodulin cDNAs of Naegleria gruberi Choi Y.J.;H.L. Park;J.H. Lee
  4. J. Cell Biol. v.105 Effects of cytochalasin and phalloidin on actin Cooper J.A.
  5. Proc. Natl. Acad. Sci. USA. v.70 Complete amino-acid sequence of actin of rabbit skeletal muscle Elzinga M.;J.H. Collins;W.M. Kuehl;R.S. Adelstein
  6. Annu. Rev. Microbiol. v.31 Cell differentiation in Naegleria gruberi Fulton C.
  7. J. Cell Biol. v.85 Cell differentiation and flagellar elongation in Naegleria gruberi Fulton C.;C. Walsh
  8. FEMS Microbiol. Lett. v.141 Molecular cloning of, and phylogenetic analysis of an actin in Naegleria fowleri Gorospe S.;R.N. Band;W.J. Kopachik
  9. J. Cell Biol. v.137 mRNAs for microtubule proteins are specifically colocalized during the sequential formation of basal body, flagella, and cytoskeletal microtubules in the differentiation of Naegleria gruberi Han J.W.;J.H. Park;M. Kim;J.H. Lee
  10. Biochem. Biophys. Acta v.17 Changes of lysine reactivities of actin in complex with DNase I. Jansco A.;L. Szilagyi;R.C. Lu.
  11. J. Cell Biol. v.106 The alpha-tubulin gene family expressed during cell differentiation in Naegleria gruberi Lai E.Y.;S.P. Remillard;C. Fulton
  12. Mol. Cell Biol. v.8 Transcriptional regulation of coordinate changes in flagellar mRNAs during differentiation of Naegleria gruberi amebae into flagellates Lee J.H.;C. Walsh
  13. J. Cell Biochem. v.6 Autoregulation of actin synthesis responds to monomeric actin levels Lyubimova A.;A.D. Bershadsky;A. Ben-Ze'ev
  14. J. Cell Biochem. v.76 Autoregulation of actin synthesis requires the 3'-UTR of actin mRNA and protects cells from actin overproduction Lyubimova A.;A.D. Bershadsky;A. Ben-Ze'ev
  15. J. Euk. Microbiol. v.44 Up-regulation of action mRNA and reorganization of the cytoskeleton in Entamoeba histolytica trophozoites Manning-Cela R.;I. Meza
  16. J. Cell Biol. v.105 New poly(A)+ RNAs appear coordinately during the differentiation of Naegleria gruberi amebae into flagellates Mar J.;J.H. Lee;D. Shea;C.J. Walsh
  17. Mol. Biochem. Parasitol. v.75 Cloning of two actin genes from Schistosoma mansoni Oliveira G.C.;W.M. Kemp
  18. FEBS. Lett. v.286 Autoregulatory control of actin synthesis in cultured rat hapatocytes Reuner K.H.;K. Schlegel;I. Just;K. Aktories;N. Katz
  19. Eur. J. Biochem. v.230 Autoregulation of actin synthesis in hepatocytes by transcriptional and posttransciptional mechanisms Reuner K.H.;M. Wiederhold;P. Dunker;I. Just;R.M. Bohle
  20. J. Mol. Biol. v.186 Organization of the actin multigene family of Dictyostelium discoideum and analysis of variability in the protein coding regions Romans P.;R.A. Firtel
  21. FEBS. Lett. v.275 Stimulation of actin synthesis in phalloidintreated cells evidence for autoregulatory control Serpinskaya A.S.;O.N. Denisenko;V.I. Gelfand;A.D. Bershadsky
  22. Biochemistry v.24 Actin-actin and actin-deoxyribonuclease I contact sites in the actin sequence Sutoh K.
  23. J. Biol. Chem. v.259 Rapid disappearance of translatable actin mRNA during cell differentiation in Naegleria Sussman D.J.;E.Y. Lai;C. Fulton
  24. EMBO. J. v.17 Nuclear export of actin: a novel mechanism regulating the subcellular localization of a major cytoskeletal protein Wada A.;M. Fukada;M. Mishima;E. Nishida
  25. Mol. Biochem. Parasitol. v.65 Cloning, sequencing and characterization of an actin cDNA in Diphyllobothrium dendriticum (Cestoda) Wahlberg M.H.;K.A. Karlstedt;G.I. Paatero