Journal of Microbiology

  • 제39권1호
  • /
  • Pages.31-36
  • /
  • 2001
  • /
  • 1225-8873(pISSN)
  • /
  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Cloning and Regulation of Schizosaccharomyces pombe Gene Encoding Ribosomal Protein S20

  • Lee, Yoon-Jong (Division of Life Sciences, Kangwon National University) ;
  • Kim, Kyunghoon (Division of Life Sciences, Kangwon National University) ;
  • Park, Eun-Hee (College of Pharmacy, Sookmyung Women′s University) ;
  • Ahn, Ki-Sup (Department of Environmental Management, Chonan College of Foreign Studies) ;
  • Kim, Daemyung (Department of Genetic Engineering, Congju University) ;
  • Lim, Chang-Jin (Division of Life Sciences, Kangwon National University)
  • 발행 : 2001.03.01
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초록

A cDNA clone encoding the ribosomal protein S20 has been isolated from the Schizosaccharomyces pombe cDNA library by colony hybridization. The insert contained in the original plasmid pYJ10 was transferred intro shuttle vector pRS316 generate plasmid pYJll. The dDNA insert of plasmid pYJll, contains 484 nucleotides and encodes a protein of 118 amino acids with a calculated mass of 13,544 daltons. The deduced amino acid sequence of S. pombe ribosomal protein S20 is very homologous with fruit fly, rat, and budding yeast counterparts. It is also homologous with Xenopus S22 ribosomal protein. S. pombe ribosomal protein S20 appears to be relatively hydruphobic except the C-terminal region. The 728 bp upstream region of the S20 gene was amplified from chromosomal DNA and transferred into the BamHI/EcoRI site of the promoterles $\beta$-galactosidase gene of the vector YEp357R, which resulted in fusion plasmid pYS20. The synthesis of $\beta$-galactosidase from the fusion plasmid appeared to be the highest in the mid-exponential phase. The S. pombe cells with the fusion plasmid grown at 35$\^{C}$ gave lower $\beta$-galactosidase activity than the cells grown at 30$\^{C}$. Computer analysis showed the consensus sequence CAGTCACA in the upstream regions of various ribosomal protein genes in S. pombe, which would be involved in the coordinated expression of small ribosomal proteins.

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참고문헌

  1. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford M.M.
  2. Gene v.200 Identification and characterization of the gene for Drosophila S20 ribosomal protein Chan H.Y.;Y. Zhang;C.J. O'Kane
  3. Biochim. Biophys. Acta v.1049 The primary structure of rat ribosomal protein S20 Chan Y.L.;I.G. Wool
  4. Biochem. Biophys. Res. Commun. v.196 Isolation of a cDNA clone, encoding the ribosomal protein S20, downregulated during the onset of apoptosis in a human leukaemic cell line Goldstone S.D.;M.F. Lavin
  5. Methods Enzymol. v.101 Yeast promoters and lacZ fusion designed to study expression of cloned genes in yeast Guarente L.
  6. Gene v.57 A ten-minute DNA preparation from yeast efficiently release autonomous plasmids for transformation of. Escherichia coli. Hoffman C.S.;F. Winston
  7. Science v.265 Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII Johnston M.;S. Andrews;R. Brinkman;J. Cooper;H. Ding
  8. J. Microbiol. v.36 Factors affecting pheromone induction of Schizosac-charomyces pombe and isolation of pheromone induction mutants Jun J.H.;Y.M. Kim;J.H. Lee;I.K. Chung;D.M. Kim
  9. Trends Genet. v.3 Developmental genetics of ribosome synthesis in Drosophila Kay M.A.;M. Jacobs-Lorena
  10. J. Biol. Chem. v.265 Nucleotide sequence and 40S subunit assembly of Xenopus laevis ribosomal protein S22 Keiper B.D.;W.M. Wormington
  11. J. Biol. Chem. v.272 Isolation, expression, and regulation of the pgr1+ gene encoding glutathione reductase absolutely required for the growth of Schizosaccharomyces pombe Lee J.;I.W. Dawes;J.-H. Roe
  12. J. Microbiol. v.38 Subcellular localization of catalase encoded by the ctt1+ gene is Schizosaccharomyces pombe Lee S.-I.;J. Lee;J.-H. Roe
  13. Gene v.45 Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusion Myers A.M.;A. Tzagoloff;D.H. Kinney;C.J. Lusty
  14. Biochim. Biophys. Acta v.1216 The primary structure of two proteins from the small ribosomal subunit of rice Nishi R.;H. Hashimoto;H. Uchimiya;A. Kato
  15. Int. J. Biochem. Cell Biol. v.27 Relationship of the expression of the S20 and L34 ribosomal proteins to polyamine biosynthesis in Escherichia coli Panagiotidis C.A.;S.C. Huang;E.S. Canellakis
  16. J. Mol. Biol. v.212 Weight matrix desciptions of four eukaryotic RNA polymerase II promoter elements derived from 502 unrelated promoter sequences Philipp B.
  17. Molecular cloning: A Laboratory Manual(2nd ed.) Sambrook J.;E.F. Fritsch;T. Maniatis
  18. Genetics v.122 A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae Sikorski R.S.;P. Hieter
  19. J. Microbiol. v.36 Isolation and caracterization of the new conditional-lethal mutation in bry4 of Schizosaccharomyces pombe by in vitro mutagenesis Song K.W.;S.J. Shin;C.F. Albright
  20. Proc. Natl. Acad. Sci. USA v.90 Complex formation between RAS and RAF and other protein kinases van Aelst L.;M. Barr;S. Marcus;A. Polverino;M. Wigler