Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Nucleotide Sequence of 7.2 kb Mitochondrial Linear Plasmid DNA in Pleurotus ostreatus

Pleurotus ostreatus 미토콘드리아의 7.2 kb 선상 플라스미드 염기서열 분석

  • 윤혜숙 (인제대학교 의생명공학대학) ;
  • 구용범 (인제대학교 의생명공학대학) ;
  • 노정혜 (서울대학교 자연과학대학 생명과학부)
  • Published : 2001.03.01
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Abstract

Two linear plasmid-like DNAs, 10.2 kb and 7.2 kb were found in the mitochondria of P. ostreatus. They have covalently linked 5'-terminal proteins in both ends. Two continuous fragments of 4.7 kb and 2.3 kb from 7.2 kb DNA were cloned and sequenced. Two long open reading frames (ORF1; 2982 bp, 993 a.a and ORF2; 2703 bp, 900 a.a) and one short open reading frame(ORF3; 771 bp, 256 a.a) were found in the 7.2 kb plasmid. The putative ORF1 and ORF2 have conserved motifs of DNA polymerases and RNA polymerases, respectively, while the ORF3 has homologous regions with phosphatase from Plasmodium, and also with adhesine from Mycoplasma.

Pleurotus ostreatus에서는 10.2 kb와 7.2 kb의 미토콘드리아 선상 플라스미드 DNA가 존재함이 발견되었다. 이 플라스미드 DNA의 양쪽 5'말단에는 단백질이 공유 결합되어있다고 알려져 있다. 최근에 P.ostreatus의 10.2 kb 미토콘드리아 선상 플라스미드 DNA를 다른 곰팡이의 선상 플라스미드 DNA에 존재하는 open reading frame(ORF)와 비교 분석하여 Podospora anserina의 플라스미드 pAL2의 DNA polymerase 및 RNA polymerase 암호부위와 유사성이 있음이 확인되었다. 본 연구에서는 7.2 kb선상 DNA를 HindIII잘라서 얻은 2조각의 절편(4.7 kb, 2.3 kb)을 클로닝하고 염기 서열을 분석하였다. 클로닝된 7 kb 절편에는 3개의 ORF,즉 ORF1(2982 bp, 993 amino acids), ORF2(2703 bp, 900 amino acids), ORF3(771 bp, 256 amino acids)가 존재함을 확인하였다 또한, 이들의 ORF를 분석한 결과, DNA polymerase와 RNA polymerase 암호부위와 유사성 이 있음을 확인하였다.

Keywords

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