한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)
- 제16권1호
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- Pages.33-36
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- 2001
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- 1229-1153(pISSN)
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- 2465-9223(eISSN)
V. parahaemolyticus ATCC 17802에 의한 Alkaline Pretense 생산조건(II)
Production Condition of Alkaline Pretense by V. parahaemolyticus ATCC 17802(II)
초록
V. parahaemolyticus를 균수로 alkaline protease 생산을 위한 배지의 초기 pH, 배양온도 그리고 배양시 진탕속도에 따른 영향을 조사하였다. 배지의 초기 pH에 따른 변화는 pH 7.6에서 60시간, 72시간 배양한 후에는8.lunit, 7.4unit로 활성이 가장 높게 나타났고, pH 7.0, 8.0 그리고 9.0에서는 활성이 크게 낫아졌으며, pH 6.0과 10.0에서는 활성이 전혀 나타나지 않았다. 배앙온도에 따른 변화를 보면 37
V. parahaemolyticus possessed an extracellular alkaline protease activity during the stationary growth phase. Various factors such as initial pH of medium, incubation temperature and shaking rate were investigated far optimizing the production of alkaline protease from V. parahaemolyticus ATCC 17802. Maximal activity of the protease was obtained when the bacteria were grown in 2% skim milk medium in 0.1M tris/HCl buffer (pH 7.6). Maximal activity of the protease was obtained when the bacteria were growls at initial pH of 7.6, incubation temperature 37