Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Effect of Dietary Fatty Acids and Vitamin E Supplementation on Antioxidant Vitamin Status of the Second Generation Rat Brain Sections

식이 지방산 및 비타민 E 보충 식이가 흰쥐의 뇌조직 부위별 항산화 비타민 농도에 미치는 영향

  • 박정화 (연세대학교 생활과학대학 식품영양학과) ;
  • 황혜진 (동의대학교 생활과학대학 식품영양학과) ;
  • 김미경 (가톨릭대학교 의과대학 예방의학교실) ;
  • 이양자 (연세대학교 생활과학대학 식품영양학과)
  • Published : 2001.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

Effects of dietary fatty acids and vitamin E on antioxidant vitamin status were studied in rat brain sections. Sources of dietary fat(10t%) were safflower oil(SO) poor in $\omega$3 fatty acid and mixed oil (MO) with computer-adjustd fatty acid ratios(AA/DHA=1.4, $\omega$6/$\omega$3=6.3, P/M/S=1.0/1.5/1, AA=2.%)with (ME) and without(MO) vitamin E(500mg/kg diet). Rats were fed the three kinds of diet from 3-4 wks prior to the conception. At the age of 3 & 9wks of the 2nd generation rat, antioxidant vitamins were measured in frontal cortex(FC), corpus striatum (CS), cerebellum(CB) and hippocampus(HP) using a multiwavelength, reverse phase gradient HPLC system. The levels of antioxidant vitamins converged to the similar value in all groups at 9 wks of age. Retinol, lycopene and cryptoxanthin levels of all experimental groups were found to be the highest in hippocampus at both 3 & 9wks of age. The levels of vitamin E appeared to be higher in the order of HP>CS-CB>FC in MO & ME. Beta-carotene and retinol showed the lowest level in hippocampus of vitamin E supplemented groups, even though vitamin E level tended to be higher in other sections. It seemed that vitamin E has an inhibitory action on the uptake of beta-carotene or acts as a preferred antioxidant to beta-carotene in certain section of the brain. By improving fatty acid balance (AA/DHA = 1.4, $\omega$6/$\omega$3=6.3, P/M/S=1.0/1.5/1, AA = 2%), the levels of vitamin E, retinol, lycopene & beta=carotene tended to be higher in MO than in SO, although crytoxanthin became lower at 3wks of age. In short, dietary fatty acids and vitamin E have different influence on antioxidant vitamin status in different rat brain sections. The higher levels of antioxidant vitamins in hippocampus should be pursued further in relation to behavioral development of rats.

Keywords

References

  1. Early hum Dev v.3 Comparative aspects of the brain growth spurt Dobbing J;Sands J
  2. J Neurochem v.35 Comparison of the fatty acid content and composition of the brain of a precocial species(guinea pig) and a nonprecocial species(rat) Patel TB;Clark JB
  3. Am J Clin Nutr v.58 Visual acuity development in healthy preterm infants-effects of marine oil supplementation Calson SE;Werkman SH;Rhodes PG;Tolley EA
  4. Lipids v.26 Effects of low intake of (n-3) fatty acids during development on brain phospholipid fatty acid composition and exploratory behavior in rats Enslen M;Milon H;Malnoe A
  5. Korean J Nutrition v.29 no.8 Effects of ω3 and ω6 fatty acids on the fatty acid composition of RBC and brain synaptosomal, microsomal and mitochondrial phospholipids and on behavioral development of rats Um YS;Chung EJ;Lee-Kim YC
  6. Am J Clin Nutr v.57 no.5 The role of essential fatty acids in neural development : implication for perinatal nutrition Crowford MA
  7. 3rd Toronto work on essential fatty acids Alpha-linolenic acid, long chanin omega-3 fatty acids and neonatal brain development Wainwrigt P
  8. J Nutr Biochem v.7 Dietary influences on membrane function : Importance in protectin against oxidative damage and disease Wisemen H
  9. Athero v.31 Do antioxidant and polyunsaturated fatty acids have a combined association with coronary atherosclerosis? Kok F;Van Poppel G;Melse E;Schouten EG;Hofman A
  10. Vitamin E : biological activity and health benefits : overview in vitamin E in health and disease Packer L;Packer L(ed.);Fuchs J(ed.);Dekker M(ed.)
  11. Korean J Nutrition v.22 no.3 Effects of dietary fatty acids and vitamin E supplementation on vitamin E levels of serum, liver and brain in chicks at different ages Chung EJ;Park YH;Lee-Kim YC
  12. Korean J Nutrition v.23 no.2 Studies on concentration of alpha-tocopherol in rat tissue and serum- I. Saponification on concentration of alpha-tocopherol in rat brain, liver and serum Park YH;Kim MK;Chung EJ;Lee-Kim YC
  13. Am J Clin Nutr v.29 Tocopherols and polyunsaturated fatty acids in human tissues Bieri JG;Everts RR
  14. N Engl J Med v.315 Serum beta-carotene, vitamin A and E, selenium and the risk of lung cancer Menks MS;;Comstock GW;Vuileumier JP;Helsing Kj;Rider AA
  15. j Natl Cancer Inst v.88 no.6 Premenopausal breast cancer risk and intake of vegetable, fruits and related nutrients Freudenheim JL;Marshall JR;;Vena JE;Laughlin R;Brasure JR;Swanson MK;Nemoto T;Graham S
  16. J Cell Biochem v.23s Nutrient antioxidants in the pathogenesis and prevention of cervical dysplasia and cancer Romney SI;Palan PR;Basu J;Mikhail M
  17. Sci v.162 Molecular basis of visual excitation Wald G
  18. Cancer Res v.43 Role of retinoids in differentiation and carcinogenesis Sporn MB;Robert AB
  19. Nature v.332 Identification of a second human retinoic acid receptor Brand N;Petkovich M;Krust A;Chambon P;de The ;H Marchio A; Tiollais P;Dejean A
  20. Nature v.330 A human retinoic acid receptor which belongs to the family of nuclear receptors Pektovich M;Brand NJ;Krust A;Chambon P
  22. Clin Biochem v.3 A fluoroemetric micro-method for fat tocopherol Hansen LG;Warwick WJ
  23. J Nutr v.113 Superoxide dismutase activity and lipid peroxidation in the rat : developmental correlations affected by manganese deficiency Zindenberg-Cheer S;Keen CI;Hurley LS
  24. Yonsei J Human Ecology v.9 A long term effect of dietary ω3 series long chain fatty acids on behavioral development Lee-Kim YC;Kim MK
  25. J Kor Neuro Assoc v.11 no.4 Effect of dietary ω3/ω6 fatty acids on the rat brain neurotransmitters Chung EJ;Lee KE;Bahang HR;Kim KH;Kim JS;Lee-Kim YC
  26. Poultry Sci v.49 Excessive dietary vitamin E : It's alleviations of hypervitaminosis A and lack of toxicity McCuaig LW;Motzok I
  27. Sci v.104 Reticulocytosis in reponse to dietary antioxidants March BE;Biely J;Coates V
  28. J Neurochem v.31 Biochemical development of rat forebrain in severe protein and essential fatty acid deficiency Karlsson I;Svennerholm I
  29. Korean J Nutrition v.31 no.5 Effects of dietary fatty acids on fatty acid pattern in developing rat brain phospholipids Um YS;Chung EJ;Lee-Kim YC
  30. Korean J Nutrition v.34 no.1 Effects of dietary fatty acid and vitamin E supplementation on antioxidant system of the second generation rat brain sections Hwang HJ;Um YS;Chung EJ;Kim SY;Lee-Kim YC
  31. Age-related alterations in antioxidative defense Matsuo M
  32. Int J Devl Neurosci v.13 no.8 Age related changes in antioxidant enzymes, superoxide dismutase, catalase, glutathione peroxidase an glutathione in different regions of mouse brain Hussain S;Slikker JRW;Ali SF
  33. J Neurosci v.20 no.1 Impaired recognition memory in monkeys after damage limited to the hippocampal region Zola SM;Squire LR;Teng E
  34. Yonsei J Euthenics v.1 Effects of supplementation of vitamin E and substitution of beta-carotene for retinyl palmtate in the diet on serum and tissue levels of vitamin E and A in rats Um YS;Lee-Kim YC
  35. J Nutr v.100 Sudies on vitamin E action : peroxidation inhibition in structural protein-lipid micelle complex derived from rat liver microsomal membranes Tinberg HM;Barber AA
  36. Doctoral thesis Studies on the status of glutamate and antioxidant system in patients with retinal ganglion cell damage Chung HY