식물 모상근 배양을 이용한 유용2차 대사산물에 관한 연구[I] - Allylisothiocyanate의 항균.살균 효과 -

On the Study of Useful Secondary Metabolites Using Plant Hairy Root Cultures -Effects of Antimicrobial and Disinfectant Activity of Allylisothiocyanate-

  • 박돈희 (전남대학교 화학공학부, 생물산업기술연구소, 촉매연구소) ;
  • 정귀택 (전남대학교 화학공학부, 생물산업기술연구소) ;
  • 양송원 (전남대학교 화학공학부, 생물산업기술연구소) ;
  • 황백 (전남대학교 생물학과) ;
  • 우희권 (전남대학교 화학과) ;
  • 이준행 (전남대학교 의과대학 미생물학교실) ;
  • 조영일 (연세대학교 화학공학과)
  • Park, Don-Hee (Faculty of Chemical Engineering, Chonnam National University) ;
  • Jeong, Gwi-Taek (Faculty of Chemical Engineering, Chonnam National University) ;
  • Yang, Song-Won (Faculty of Chemical Engineering, Chonnam National University) ;
  • Hwang, Baik (Dept. of Biology, Chonnam National University) ;
  • Woo, Hee-Gweon (Dept. of Chemistry, Chonnam National University) ;
  • Rhee, Joon-Haeng (Dept. of Microbiology, Medical School, Chonnam National University) ;
  • Joe, Yung-Il (Dept. of Chemical Engineering, Yonsei University)
  • 발행 : 2001.08.01

초록

고추냉이 뿌리에서 추출된 정유(精油) 물질 중에 약 80%를 차지하고 있는 것이 allylisothiocyanate(AIT)로 알려지고 있다. Allylisothiocyanate 물질은 고추냉이 식물의 2차 대사산물로서 고부가가치를 가질 수 있다. 본 연구는 고추냉이의 allylisothiocyanate를 대량으로 생산하여 산업화하기 위한 기초 연구로서 allylisothiocyanate 물질의 항균과 살균효과를 관찰하였다. 결론적으로 실험에 사용한 미생물에 대한 allylisothiocyanate 농도를 50 ppm 정도 이상 첨가하였을 때 최소 15시간 동안은 증식억제 효과가 있음을 알았다.

It is known as the allylisothiocyanate which is extracted from Wasabia koreana\\\\\\\\`s root. It contains 80% of the oil refining material. The allylisothiocyanate as secondary metabolites of Wasabia koreana plant is a possibility of having the high value which is useful. The research observed the allylisothiocyanate material as the basic study for industrializing to make its mass product. Finally, it shows that the antimicrobial and disinfectant effect against the microbe incubated adding 50 ppm allylisothiocyanate for 15 hours.

키워드

참고문헌

  1. Proc.Intl.Plant.Prop.Soc. v.36 Production of Wasabia Japonica in Japan John,M.Follett
  2. Agric,Bio.Chem v.43 no.11 Studies on the myrosinase from Wasibia Japanica Ohtsuru,M.;H.Kawatani
  3. Korean J.Nutr. v.18 no.4 Volatile Compounds Characterizing the Flavor of Korean Horseradish Roots Lim,I.S.;M.S.Lee
  4. Korean J.Crop.Sci. v.42 no.3 Allyisothiocyanate Content in Different Plant Parts if Wasabia japonica mastum Lee,S.W.;J.S.Seo;S.D.Kim;Y.H.Kim;S.N.Yu;D.Y.Kim
  5. Mechanisms of Ageing and Development v.116 Antiplatelet and anticancer isothiocyanates in Japanese domestic horseradish,Wasabi Yasujiro,Morimitsu,et.al.
  6. Korean J.Dietary Culture v.4 no.4 Effect of the Volatile Constituents of Mugwort Seed Extract on the Growth of Microorganism Jung,B.S.;B.K.Lee;S.T.Shim;J.K.Lee
  7. Antimicrobials in Foods,Second Edition Davidson,P.Michael;Alfrred Larry Branen
  8. The Merck Index Centenial Edition
  9. Quantitative Bioassay David Hawcroft et.al.
  10. Applied and Enviromental Microbiology Quantitative Determination of Infinite Inhibition Concentrtions of Antimicrobial Agents Marwan,Aref G;Charles W.Nagel
  11. Methods for Evaluation,In Antimicrobials in Foods Parish,Mickey E.;P.Michael Davidson;Michael Davidson(ed.);Alfred Larry Branen(ed.)