공초점 광학현미경에서의 side-lobe 감소방안에 대한 연구

A study on the effect of side-lobe on axial image of confocal microscope

  • 김억봉 (제주대학교 물리학과, 기초과학연구소) ;
  • 류재연 (제주대학교 물리학과, 기초과학연구소) ;
  • 최치규 (제주대학교 물리학과, 기초과학연구소) ;
  • 김두철 (제주대학교 물리학과, 기초과학연구소) ;
  • 유영훈 (제주대학교 물리학과, 기초과학연구소)
  • 발행 : 2001.10.01

초록

공초점 현미경에서 광축 방향의 상을 얻을 때 잡음으로 작용하는 side-lobe의 영향을 조사하였다. 이러한 side-lobe는 Herschel 조건을 만족시킴으로써 최소화시킬 수 있다. 본 연구에서는 side-lobe에 대한 영향을 전산 시늉하였고, 이 결과를 실험에 적용하여 Herschel 조건에 의해 위상 보정을 함으로써 side-lobe가 최소화됨을 확인하였다.

The effect of side-lobe on axial image is investigated. We show, using computer simulation and experimentally, that an optimized axial response can be achieved by minimized side-lobe according to the Herschel condition and that the appearance of sidelobes is decreased by balancing phase error.

키워드

참고문헌

  1. Confocal Scanning Optical Microscopy And Related Imaging System T. R. Corle;G. S. Kino
  2. Measurement Science & Technology v.11 no.12 Surface profilometry with a fibre optical confocal scanning micro-scope L. S. Yang;G. Y. Wang;J. G. Wang;Z. Z. Xu
  3. Review of Scientific Instruments v.71 no.11 A simple submicron confo-cal microscope with fiberoptic output I. K. Ilev;R. W. Waynant
  4. Opt. Lett. v.25 no.8 Geometrical cross-sectional imaging by a heterodyne wavelength-scanning interference confocal microscope T. Fukano;I. Yamaguchi
  5. Optics Comm. v.174 no.1;4 Confocal multipoint multiphoton excitation microscope with microlens and arrays K. Fujita;O. Nakamura;T. Kanko;M. Oyamada;T. Taka-matsu;S. Kawata
  6. J. Appl. Phy. v.84 no.8 Three-dimensional super-resolution with a 4pi-confocal micro-scope using image restoration M. Schrader;S.W. Hell;H.T.M. Vandervoort
  7. Opti. Eng v.38 no.6 High-speed surface measure-ment using a nonscanning multiple-beam confocal micro-scope M. Ishihara;H. Sasaki
  8. J. Mod. Opti. v.46 no.4 Effects of system geometry on the axial response of the fibreoptical confocal microscope M. D. Sharma;C. J. R. Sheppard
  9. Japan. J. Appl. Phy. v.37 no.4B Resolution issues in con-focal magnetooptic scanning laser microscopy P. W. Nutter;C. D. Wright
  10. U.S. patent #3013467 Microscopy apparatus M. Minsky
  11. J. Cell. Sci. v.94 Confocal scanning optical microscopy and its application for bilogical specimens D. M. Shotton
  12. Multidi-mensioal Microscopy P. C. Cheng;T. H. Lin;W. L. Wu;J. L. Wu
  13. Appl. Phys. Lett. v.38 no.11 Effect of high angels of convergence on V(Z) in the scanning acoustic microscope C.J.R. Sheppard;T. Wilson
  14. Opt. Comm. v.88 no.2 Axial imaging through an aberrating layer of water in confocal microscopy C. J. R. Sheppard;M. Gu
  15. Optik v.87 no.1 Effect of aberrating layers and tube length on confocal imaging properties C.J.R. Sheppard;C.J. Cogswell
  16. Appl. Opt. v.30 no.25 Aberration compensation in confocal imaging systems C. J. R. Sheppard;M. Gu
  17. Appl. Opt. v.27 no.22 Aberrations in high aperture conven-tional and confocal imaging systems C. J. R. Sheepard
  18. Signal Processing Using Optics G. Boone
  19. Appl. Opt. v.35 no.1 Confocal theta fluorescene microscopy with annular apertutes S. Lindek;C. Cremer;E. H. K. Stelzer
  20. Appl. Opt. v.33 no.4 Influ-ence of spherical aberration on axial imaging of confocal reflection microscopy C. J. R. Sheppard;M. Gu;K. Brain;H. Zhou
  21. Opt. Lett v.20 no.10 Simple binary optical elements for aberration correction in confo-cal microscope C. K. Sieracki;C. G. Levey;E. W. Hansen