Antimutagenic and Antioxidative Effects of Seafood Bun with Kimchi

김치 해물만두의 항돌연변이 및 항산화 효과

Antimutagenic, antioxidative and nitrite-scavenging effects of seafood bun were investigated. Each samples were extracted with methanol. Aflatoxin Bl(AFB1) was used as mutagen. Seafood bun added 20% kimchi(5mg/plate) reduced mutagenicity of AFB1 for Salmonella typhimurium TA and YG 1024 to 47% and 61%, rrespectivery. The reduction rate of seafood bun added 20% kimchi was higher relative to that of other samples. Mydrogen donating activity in all buns showed over 50% and seafood bun added 20% kimchi was higher than others, but the activity was lower than that of 0.1% BHT. The peroxide value for linoleic acid increased during the storage, the values in seafood bun added 20% kimchi was higher than others, but the activity was lower than that of 0.1% BHT. The peroxide value for linoleic acid increased during the storage, the values in seafood bun added 20% kimchi was significantly lower and the values in other buns were a lower than that of control, the values in all of the samples were higher than that in 0.1% BHT. Among the samples tested, the TBA value in the seafood bun added 20% kimchi for liver homogenate of rat was the lowest. Nitrite-scavenging effect in all the samples tested was higher than 50%.

김치 첨가 해물만두의 생리기능성을 조사하고자 시판 만두, 해물만두 및 20% 김치첨가 해물만두 추출물에 대한 항돌연변이, 항산화 효과 및 아질산염 소기능에 대하여 조사하였다. 간접돌면변이원으로 알려진 aflatoxin B$_1$을 첨가한 Salmonella typhimurium TA 100 및 YG 1024에 만두 추출물을 5 mg/plate 농도로 처리한 후 항돌연변이 효과를 조사한 결과 20% 김치첨가 해물만두의 돌연변이 저해율이 47% 및 61%로 가장 높게 나타났으며, 수소공여능은 모두 50% 이상의 활성을 나타내었고, 시판만두, 해물만두 및 20% 김치첨가 해물만두의 순으로 높게 나타났으나, 모두 0.1%의 BHT 첨가군 보다는 낮았다. Linoleic acid에 대한 과산화물가는 저장기간이 지남에 따라 대조군의 과산화물가는 크게 증가한 반면 20% 김치첨가 해물만두 첨가군에서는 대조군에 비하여 매우 낮게 나타났으며, 시판 및 해물만두 추출물의 경우는 약간 낮게 나타났으나 0.1% BHT 첨가군보다는 모두 높았다. 횐쥐의 liver homogenate에 만두추출물을 처리한 후 TBA가를 조사한 결과 20% 김치첨가 해물만두 추출물 처리구가 가장 낮게 나타났다. 아질산염 소거능은 모두 50% 이상이었다.