Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Cometabolism of Trichloroethylene by a Phenol-Degrading Bacterium, Pseudomonae sp. EL-04J

페놀분해세균인 Pseudomonas sp. EL-04J에 의한 Trichloroethylene의 공동대사

  • 김호성 (부산대학교 미생물학과) ;
  • 박근태 (부산대학교 미생물학과) ;
  • 손홍주 (밀양대학교 생물공학과) ;
  • 박성훈 (부산대학교 응용화학공학부 및 환경기술산업개발연구소) ;
  • 이상중 (부산대학교 미생물학과)
  • Published : 2001.10.01
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Abstract

Pseudomanas sp. EL-04J was previously isolated from phenol-acclimated activated sludge. This bacterium was capable of degrading phenol and cometabolizing trichloroethylene (TCE). After precultivation in the mineral salts medium containing phenol as a sole carbon source, Pseudomonas EL-04J degraded 90% of TCE $25 \mu\textrm{M}$ within 20 hours. Thus, phenol-induced Pseudomonas sp. EL-04J cells can bdegrade TCE. Followsing a transient lag period, Pseudomonas sp. EL-04J cells degraded TCE at concentrations of at least $250 \mu\textrm{M}$ with no apparent retardation in rate, but the transformance capacity of such cells was limited and depended on the cell concentration. The degradation rate of TCE followed the Michaelis-Menten kinetic model. The maximum degradation ratio ($V_{max}$) and saturation constant ($K_{m}$) were $7nmo {\ell}/min{\cdot}mg$ cell protein and $11 \mu\textrm{M}$, respectively. Cometabolism of TCE by phenol fed experiment was evaluated in $50m {\ell}$ serum vial that contained $10m {\ell}$ of meneral sals medium supplemented with $10 \mu\textrm{M}$ TCE degradation was inhibited in the initial period of 1 mM phenol addition, but after that time Pseudomonas sp. EL-04J cells degraded TCE and showed cell growth.

Keywords

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