Application of Oxygen Uptake Rate Measured by a Dynamic Method for Analysis of Related Fermentation Parameters in Cyclosporin A Fermentation:Suspended and Immobilized Cell Cultures

  • Chun, Gie-Taek (Division of Life Science, College of Natural Sciences, Kangwon National University) ;
  • Agathos, S.N. (Unit of Bioengineering, Catholic University of Louvain, Place Croix du sud,)
  • 발행 : 2001.12.01

초록

Experimental data for the on-line estimation of cell concentration and growth rate are presented. For this purpose, we utilized the on-line calculation of the oxygen uptake rate (OUR), which was derived from a liquid phase dynamic mass balance for the oxygen during the active growth phase in cyclosporin A (CyA) fermentation. The cell yield coefficient, based on the oxygen $(Y_{x/o})$for both suspended and immobilized cells of Tolypocladium inflatum, was estimated as $1.9 gDCW/gO_2$ from a very good linear correlation between the cell mass produced and the total oxygen consumed. The calculated yield showed a good agreement with the value of $(Y_{x/o})$ generated from the correlation between the cell growth rate and the oxygen uptake rate. In addition, further experimental data are given, which were also applied to determine the specific oxygen uptake rate of T. inflatum cells during the exponential phase of CyA fermentation. A theoretical basis for the analysis of these fermentation parameters is also provided.

키워드

참고문헌

  1. Biochemical Engineering Fundamentals(2nd ed.) Bailey, J. E.;D. F. Ollis
  2. J. Biol. Chem. v.262 Enzymatic synthesis of cyclosporin A Billich, A.;R. Zocher
  3. Cyclosporin, Progress in Allergy v.38 Cyclosporin and its future Borel, J. F.;Borel, J. F.(ed.)
  4. Methods in Microbiology v.1 Calam, C. T.
  5. Biotechnol. Bioeng. v.37 Comparative studies of physiological and environmental effects on the production of cyclosporin A in suspended and immobilized cells of Tolypocladium inflatum Chun, G.-T.;S. N. Agathos
  6. Biotechnology. A Textbook of Industrial Microbiology(2nd ed.) Crueger, W.;A. Crueger
  7. Bioprocess Engineering Principles Doran, P. M.
  8. Enzyme Microb. Technol. v.12 Fermentation pathway kinetics and metabolic flux control in suspended and immobilized Saccharomyces cerevisiae Galazzo, J. L.;J. E. Bailey
  9. Biotechnol. Bioeng. v.25 Confining mycelial growth to porous microbeads: A novel technique to alter the morphology of non-Newtonian mycelial cultures Gbewonyo, K.;D. I. C. Wang
  10. Biotechnol. Bioeng. v.25 Enhancing gas-liquid mass transfer rates in non-Newtonian fermentaions by confining mycelial growth to microbeads in a bubble column Gbewonyo, K.;D. I. C. Wang
  11. Eur. J. Appl. Microbiol. v.14 Directed biosynthesis of cyclosporins Kobel, H.;R. Traber
  12. J. Microbiol. Biotechnol. v.9 Heterologous expression of Streptomyces albus genes linked to an intergrating element and activation of antibiotic production Kwon, H.-J.;S.-Y. Lee;S.-K. Hong;U. M. Park;J.-W. Suh
  13. J. Antibiotic v.42 Cell free synthesis of new cyclosporins Lawen, A.;R. Traber;D. Geyl;R. Zocher;H. Keinkauf
  14. Korean J. Biotechnol. Bioeng. v.11 Effect of medium components on the production of cyclosporin A by immobilized fungal cell, Tolypocladium inflatum Lee, T. H.;Y. K. Chang;G. T. Chun
  15. J. Gen. Microbiol. v.50 The influence of maintenance energy and growth rate on the metabolic activity, morphology and condiation of Pencillium chrysogenum Righelato, R. C.;A. P. J. Trinci;S. J. Pirt;A. Peat
  16. J. Microbiol. Biotechnol. v.10 New polyene macrolide antibiotics from Streptomyces sp. M90025 Seo, Y.;K. W. Cho;H.-S. Lee;T.-M. Yoon;J. Shin
  17. Bioprocess Engineering Sheler, M. L.;F. Kargi
  18. Manual of Industrial Microbiology and Biotechnology (2nd ed.) Instrumentation of small-scale bioreactors Sonnleitner, B.
  19. Principles of Fermentation Technology(2nd ed.) Stanbury, P. F.;A. Whitaker;S. J. Hall
  20. J. Antibiotic v.42 Cyclosporins-new analogue by precursor directed biosynthesis Traber, R.;H. Hofmann;H. Kobel
  21. Microbial Growth Dynamics, Special Publications of The Society of General Microbiology v.28 Kinetics of secondary metabolic production Trilli, A.
  22. Fermentation and Enzyme Technology Wang, D. I. C.;C. L. Cooney;A. L. Demain;P. Dunnill;A. E. Humphrey;M. D. Lilly
  23. Manual of Industrial Microbiology and Biotechnology Bioinstrumentation and computer control of fermentation process Wang, H. Y.;Demain, A. L.(ed.);N. A. Solomon(ed.)
  24. Biotechnol. Bioeng. v.37 Oxygen uptake by entrapped hybridoma cells Wohlpart, D.;J. Gainer;D. Kirwan
  25. AIChE J. v.24 Real-time estimation of aerobic batch fermentation biomass concentration by component balancing Zabriskie, D. W.;A. E. Humphrey
  26. A. Phytochem. v.23 Biosynthesis of cyclosporin Zocher, R.;N. Madry;H. Peeters;H. Kleinkauft