Journal of Microbiology and Biotechnology

  • 제11권6호
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  • Pages.973-978
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  • 2001
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Molecular Cloning and Expression of a Sodium-Driven Flagellar Motor Component Gene(motX) from Vibrio fluvialis

  • Park, Je-Hyeon (Department of Bitechnology and Bioengineering, Pukyong National University) ;
  • Lee, Jong-Hee (Department of Bitechnology and Bioengineering, Pukyong National University) ;
  • Kim, Young-Sook (Department of Food Science and Technology, Yangsan College) ;
  • Hong, Yong-Ki (Department of Bitechnology and Bioengineering, Pukyong National University) ;
  • Kong, In-Soo (Department of Bitechnology and Bioengineering, Pukyong National University)
  • 발행 : 2001.12.01
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초록

The bacterial flagellar motor is a molecular machine that couples proton or sodium influx to force generation, mostly for driving rotation of the helical flagellar filament. In this study, we cloned a gene (motX) encoding a component of the sodium-driven flagellar motor from Vibrio fluvialis. The nucleotide sequence of the motX gene, composed of 633 bp and 211 amino acid residues, was determined. Overexpression of the motX gene in Escherichia coli using a strong promoter induced growth inhibition and cell lysis. The lethal effect of E. coli was suppressed by adding amiloride, as a potent inhibitor for the sodium channel. Electron microscopic observation of the expressed protein indicated that MotX protein induced by isopropyl ${\beta}$-D-thiogalactopyranoside caused the lysis of host cell.

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