Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Molecular Cloning and Expression of a Sodium-Driven Flagellar Motor Component Gene(motX) from Vibrio fluvialis

  • Park, Je-Hyeon (Department of Bitechnology and Bioengineering, Pukyong National University) ;
  • Lee, Jong-Hee (Department of Bitechnology and Bioengineering, Pukyong National University) ;
  • Kim, Young-Sook (Department of Food Science and Technology, Yangsan College) ;
  • Hong, Yong-Ki (Department of Bitechnology and Bioengineering, Pukyong National University) ;
  • Kong, In-Soo (Department of Bitechnology and Bioengineering, Pukyong National University)
  • Published : 2001.12.01
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Abstract

The bacterial flagellar motor is a molecular machine that couples proton or sodium influx to force generation, mostly for driving rotation of the helical flagellar filament. In this study, we cloned a gene (motX) encoding a component of the sodium-driven flagellar motor from Vibrio fluvialis. The nucleotide sequence of the motX gene, composed of 633 bp and 211 amino acid residues, was determined. Overexpression of the motX gene in Escherichia coli using a strong promoter induced growth inhibition and cell lysis. The lethal effect of E. coli was suppressed by adding amiloride, as a potent inhibitor for the sodium channel. Electron microscopic observation of the expressed protein indicated that MotX protein induced by isopropyl ${\beta}$-D-thiogalactopyranoside caused the lysis of host cell.

Keywords

References

  1. J. Bacteriol. v.179 Prtative channel components for the fastoratating sodium-driven flagellar motor of a marine bacterium Asai, Y.;S. Kojima;H. Kato;N. Nishioka;I. Kawagishi;M. Nomma
  2. J. Bacteriol. v.178 Effect of viscosity on swimming by the lateral and polar glagella of Vibrio alginolyticus Atsumi, T.;Y. Maekawa;T. Yamada;I. Kawagishi;Y. Imae;M. Homma
  3. Nature v.355 Polar and lateral flagellar motors of marine Vibrio are driven by different ion-motive forces Atsumi, T.;L. McCarter;Y. Imae
  4. J. Bacteriol. v.172 Specific inhibitioin of the M$Na^+$-driven flagellar motors of alkalophilic Bacillus strain by the amiloride analog phenamil Atsumi, t.;S. Sugiyama;E. J. Cragoe;Y. Imae
  5. Infect. Immun. v.62 Identification of surface0exposed outer membrane antigens of Helicobacter pylori Doig, P.;T. J. Trust
  6. Microbiol. Immunol. v.43 Sippression by the DNA fragment of the motX promoter region on long flagellar mutants of Vibrio alginolyticus Furuno, M.;N. Nishioka;I. Kawagishi;M. Homma
  7. Proc. Natl. Acad. Sci. USA v.96 Mutations conferring resistance to phenamil and amiloride, inbitors of sodium-driven motility of Vibrio parahaemolyticus Jaques, s.;Y. K. Kim;L. McCarter
  8. J. Bacteriol. v.177 Isolation of the polar and lateral flagellum-defective mutants in Vibio alginolyticus and identification of their flagellar drviving energy sources Kawagishi, I.;Y. Maekawa;T. Atsumi;M. Homma;Y. Imae
  9. Biochem. Biophys. Acta v.1384 Isolation and sequence analysis of metalloprotease gene from Vbrio mimicus Lee, J. H.;G. T. Kim;J. Y. Lee;H. K. Hun;J. H. Yu;I. S. Kong
  10. J. Microbiol. Biotechnol. v.9 Production of a fibrolytic enzyme in bioreactor culture by Bacillus subtilis BK-17 Lee, J.;S. Oark;W. A. Choi;K. H. Lee;Y. K. Jeong;I. S. Kong;S. Park
  11. J. Microbiol. Biotechnol. v.9 Characterization of campylobacters newly isolated from swine gastric mucosa Lee, Y.;J. Lee;H. Cho;E. Shin;J. H. Park
  12. J. Bacteriol. v.172 Ion selectivity of the Vbrio alginolyticus flagellar motor Liu, J. Z.;M. Dapice;S. Khan
  13. J. Bacteriol. v.176 MotY, a component of the sodium-type flagellar motor McCarter, L.
  14. J. Bacteriol. v.176 MotX, the channel component of the sodium-type flagellar motor McCarter, L.
  15. Mol. Microbiol. v.4 Surface-induced swarmer cell differentiation of Vibrio parahaemolyticus McCarter, L.;M. Silverman
  16. J. Mol. Biol. v.251 High speed rotation and speed stability of the sodium-driven flagellar motor in Vibrio alginolyticus Muramoto, K.;I. Kawagishi;S. Kudo;Y. Magariyama;Y. Imae;M. Homma
  17. J. Bacteriol. v.178 Cloning and characterization of motY, a gene coding for a component of the sodium0driven flagellar motor in Vibrio alginolyticus Okunishi, I.;I. Kawagishi;M. Homma
  18. J. Microbiol. Biotechnol. v.10 Overexpression and characterization of Vibrio mimicus metalloprotease Shin, S. Y.;J. H. Le;S. H. Huh;Y. S. Park;J. M. Kim;I. s. Kong
  19. Histochem. J. v.25 Rapid cold fization of tisseu sample by microwave irradiation for use in electtron microscopy Wagenaar, F.;G. L. Kok;J. M. B Davies;J. M. A. Pol
  20. J. bacteriol. v.181 Functional interaction between pomA and pomB, the sodium-driven flagellar motor components fo Vibrio alginolyticus Yorimitsu, T.;K. Sato;Y. Asai;I. Kawagishi;M. Homma