Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Reactivity and Stability of Lignostilbene-$\alpha$, $\beta$-Dioxygenase-I in Various pHs, Temperatures, and in Aqueous Organic Solvents

  • Makoto, Niwa (Pharmacolinetics Group, Nippon Kayaky Co., Ltd.) ;
  • Kamoda, Shigehiro (Department of Biomaterial Sciences, Graduate School of Agriculture and Agricultural Life Sciences, The University of Tokyo) ;
  • Saburi, Yoshimasa (Department of Biomaterial Sciences, Graduate School of Agriculture and Agricultural Life Sciences, The University of Tokyo)
  • Published : 2001.10.01
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Abstract

The reactivity and stability of purified Lignostilbene- ${\alpha}$,${\beta$}-dioxygenase (LSD)-I were examined. Its optimum temperature was $50^{\circ}C$ at pH 8.5, but it was stable only up to $30^{\circ}C$. The activity of LSD-I increased 12-fold by $30\%$, with increased $V_{max}$ and lowered $K_m.$ LSD-I was stable in 10% methanol.

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