Effect of Electromagnetic Fields on Growth of Human Cell Lines

  • Oh, Se-Jong (Division of Food Biotechnology, Kangwon National University) ;
  • Lee, Mi-Kyung (Division of Food Biotechnology, Kangwon National University) ;
  • Lee, Seo-Ho (Division of Food Biotechnology, Kangwon National University) ;
  • Lee, Jin-Ha (Division of Food Biotechnology, Kangwon National University) ;
  • Kim, Dai-Jong (Division of Food Biotechnology, Kangwon National University) ;
  • Park, Young-Shik (Division of Food Biotechnology, Kangwon National University) ;
  • Lee, Hyeon-Yong (Division of Food Biotechnology, Kangwon National University)
  • 발행 : 2001.10.01

초록

High Electromagnetic Field (EMF) with an intensity of 1 mT (Tesla) inhibited the growth of both human normal lung and immune T cell down to $20-30\%$, compared to that of an unexposed case. The human T-cells, Jurkat, were more severely affected by EMF than the human lung cells, which showed a relatively slow cell growth and substantial releas of $Ca^+2$ (3.5 times higher than the human T-cells). However, the growth of hepatoma carcinoma, Hep3B, was enhanced by twice that of an unexposed case. The EMF intensity and exposure time did not affect the growth of the cancer cells very much, while it significantly affected the growth of normal cells. Accordingly, it is possible that EMFs may play a role in the initiation of cancer. The EMFs disturbed the signal transduction and membrane systems, such that a five times higher amount of PKC-${\alpha}$ was released from the cell membrane than in the control. Extended exposure to EMFs, for more than 48 hours, also led to 1 $90\%$ necrotic death pattern from apoptotic cell death. Finally, EMF at an intensity of 1mT with a 24-T exposure promoted the differentiation of HL-60 cells to monocytes/macrophages, possibly causing potential acute leukemia.

키워드

참고문헌

  1. Nature v.360 EMF report draws fire Anderson, G.
  2. Lancet v.342 Electromagnetic fields and childhood cancer Ahlbom, A.;M. L. Feychting;M. Koskenvuo;J. H. Olsen;E. Pukkala;D. Verkasalo
  3. Carcinogenesis v.8 The effects of low energy 60Hz environment upon growth related enzyme decarboxylase Byus, C. V.;S. F. Pieper;W. R. Adey
  4. Bioelectrochem. Bioenerg. v.33 Correlation between the amplitude of plasma membrane fluctuation and the response to cells Broude, N. L.;R. Karabakhistian;N. A. Shatls;A. S. Henderson
  5. Bioelectrochem. Bioenerg. v.48 Problems of weak electromagnetic field affects in cell biology Berg, H.
  6. Biochem. Pharmacol. v.55 Inhibition of the membrane translocation and activation of protein kinase C and potentiation of doxorubicin induced apoptosis of hepatocellular carcinoma cells Cheng, A. L.;S. E. Chuang;R. L. Fine;K. H. Yal;C. M. Liao;D. S. Chen
  7. Blood v.85 All trans retinoic acid as a differentiating agent in the treatment of acute promyleocytic leukemia Degos, L. K.;H. F. Dombret;C. Y. Chomienne;M. T. Daniel;J. M. Micea;C. W. Chastang;P. D. Fenaus
  8. Cultivation of Animal Cells Freshney, R. I.
  9. Calcified Tissue Int. v.55 Combined magnetic fields increased net calcium flux in bone cells Futzimmons, R. J.;J. T. Rayby;F. P. Magee;D. J. Baylink
  10. Cell Stress Cheprones v.3 Magnetic field stress induces expression of hsp 70 Goodman, R. P.;M. O. Blank
  11. Bioelectrochem. Bioenerg. v.25 Transcription and transduction in cells exposed to extremely low frequency electromagnetic fields Goodman, R.;H. A. Shirley
  12. Am. J. Epidemiol. v.143 Childhood Brain tumor occurrence in relation to residential power line configurations Gurney, J. G.;B. A. Mueller;S. Davis;S. M. Schwartz;R. G. Stevens;K. J. Kopechy
  13. J. Microbiol. Biotechnol. v.9 The changes of growth patterns and the production of Brain-Derived Neurotrophic Factors (BDNFs) in perfusion cultivation of human neuroblastoma cells Hong, J. S.;J. N. Lee;S. H. Kim;K. Y. Park;J. S. Cho;H. Y. Lee
  14. J. Natl. Cancer Inst. v.84 Overexpression of the MDR-1 gene and P-glycoprotein in human hepatomacellular carcinoma Huang, C. W.;M. E. Wu;G. J. Xu;D. D. Li;H. F. Chen;H. L. Jiang;J. A. Gu
  15. Bioelectrochem. Bioenerg. v.44 Biological and technical variables in myc expression in HL 60 cells exposed to 690 Hz electromagnetic fields Jin, M.;H. L. Lin;M. K. Opler;S. Maurer;M. O. Blank;R. P. Goodman
  16. J. Biol. Chem. v.269 Activation of Str-like p56/p53 tyrosine kinase by ionizing radiation Kharbanda, S.;Z. M. Yuan;F. L. Rubin;R. Weichselbaum;D. G. Kufe
  17. Bioelectromagnetics v.12 Possible mechanism for the influence of weak magnetic fields on biological systems Lednev, V. V.
  18. Occup. Environ. Med. v.53 Epidemiological appraisal of studies of residential exposure to power frequency magnetic fields and adult cancers Li, C. Y.;G. Theriault;R. S. Lin
  19. Bioelectrochem. Bioenerg. v.36 Electric and magnetic noise holds the 60 Hz magnetic field enhancement of steady static c-myc transcript levels in human leukemia cells Lin, H.;R. Goodman
  20. J. Radiol. Protect. v.14 Exposure to power-frequency magnetic fields in the home Merchant, C. J.;D. C. Renew;J. Swanson
  21. J. Cell Physiol. v.131 Magnesium deprivation inhibits the expression of differentiation related phenotypes in human promyelocytic leukemia HL-60 cells Okazaki, T.;T. G. Mochizuka;M. Tashima;H. I. Sawada;H. A. Uchino
  22. Biotechnol. Bioeng. v.44 Cell cycle kinetics and monoclonal antibody production of hybridoma cells during perfusion culture Park, S. H.;D. Y. Ryu
  23. NIEHS Working Group Report Assessment of health effects from exposure to power line frequency electric and magnetic fields Portier, C. L.;M. Volfe
  24. Bioelectrochem. Bioenerg. v.40 Evaluating the biological aspects of in vitro studies in bioelectromagnetics Saffer, J. D.;J. L. Phillips
  25. Am. J. Epidemiol. v.128 Case control study of childhood cancer and exposure to 60 Hz magnetic fields Savitz, D. A.;H. Wachtel;F. A. Barnes;E. M. John;J. G. Tvrdik
  26. J. Immunol. v.147 The signal transduction pathway involved in the migration induced by a monocyte chemotatic cytokine Sozzani, S. W.;W. Luini;M. S. Molino;P. Jiliket;C. A. Cerletti;A. S. Mantovani
  27. J. Am. Med. Assoc. v.268 Epidemiological and laboratory studies of power frequency electric and magnetic fields Sagan, L. A.
  28. J. Biol. Chem. v.269 Dissociation of phorbol esters leads to immediate redistribution to the cytosol of protein kinase C alpha and delta in mouse kernocytes Szallasi, Z. D.;C. B. Smith;P. M.;Blumberg
  29. Anticancer Res. v.15 Endonuclease activity and induction of DNA fragmentation in human myelogenous leukemic cell lines Shiota, Y. F.;H. Sakgami;N. Kuribayashi;M. Ilda;T. Sakagami;M. Takeda
  30. Radiation Res. v.144 Short exposure to 60 Hz magnetic fields do not alter MYC expression in HL60 Saffer, J. D.;S. J. Thurston