Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Cytotoxic Effects and Components of Lipid Fractions from Soybean Products on Cancer Cell Lines

대두식품 지질추출물의 세포독성 및 지질성분분석

  • 송성광 (경성대학교 식품공학과) ;
  • 김광혁 (고신대학교 의과대학 미생물학교실) ;
  • 김희숙 (경성대학교 식품공학과)
  • Published : 2001.12.01
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Abstract

The cytotoxic effects of lipid extracts from soybean products were studied using K562 human leukemia cell, Yac1 mouse leukemia cell and S 180 mouse sarcoma cell. Total lipids from soybean powder, soybean curd residue and doenjang were extracted with chloroform/methanol (2 : 1) and water saturated butanol, consecutively, and fractionated into acetone supernatants (AS fraction) and acetone precipitates (AP fraction) by adding excess acetone. AS fraction of doenjang lipids showed the strongest cytotoxic effects on K562, Yac1 and S180 cancer cells, whereas each lipid fraction of soybean curd residue also showed relatively weak cytotoxic effects on cancer cells but soybean powder did not. AS and AP fractions of doenjang contained more free fatty acids than those of soybean curd residue and soybean. And when lipid fractions were digested with 0.4 N KOH/methanol, doenjang lipid fractions showed to contain some alkali-stable substances which showed positive reaction with ninhydrin solution on silica TLC separation.

대두식품들로부터 추출한 지질분획들이 사람의 K562 백혈구암세포, mouse의 Yak1 백혈구암세포 및 S180 고형암세포에 미치는 세포독성 효과를 MTT assay법으로 조사하였다. 대두, 비지 및 된장의 총지질을 chloroform/methanol(2 : 1) 및 물포화 butanol로 추출하고 20배 용량의 acetone을 가하여 아세톤상층액(AS 획분) 및 아세톤침전물(AP 획분)을 얻었다 된장지질의 AS 획분 및 AP 획분이 실험에 사용한 K562, Yac1 및 S180암세포에 가장 강한 세포독성을 일으켰으며 비지 의 AS 및 AP 획분 역시 된장보다는 약하지만 세포독성을 나타냈으나 대두의 지질획분들은 세포독성효과를 나타내지 않았다. 된장지질의 AS 및 AP분획의 지질조성을 보면 대두 및 비지에 비하여 많은 양의 유리지방산이 함유되어 있었다. 또한 지질획 분들을 0.4 N KOH/methanol로 약알카리 분해하고 silica gel TLC로 분석한 결과, 된장의 지질획분에서는 대두 및 비지에서 보이지 않았던 알카리에 안정하고 ninhydrin에 양성인 물질들이 여러개 나타났으며 이들은 유리 sphingoid base 및 psycusin으로 추정되었다.

Keywords

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