Effects of the Antioxidants on the Diabetic Complications

당뇨병성 합병증에 대한 항산화제 효과

  • 김진영 (건국대학교 수의과대학 내과학교실) ;
  • 이병한 (건국대학교 수의과대학 내과학교실) ;
  • 정병현 (건국대학교 수의과대학 내과학교실)
  • Published : 2001.12.01

Abstract

These studies were performed to investigate the effect of antioxidants, melatonin (MLT) and ascorbic acid (AA), on the diabetic complications. In the experiment, twenty-four Sprague Dawley rats were divided into four groups; Group 1: no treatment (control) (n=6), Group 2: Streptozotocin (STZ; 65 mg/kg b.w. ip) treatment (n=6), Group 3: MLT (10 mg/kg b.w. ip) treatment (n=6), Group 4: ascorbic acid (AA: 40 mg/kg b.w.ip) treatment (n=6). Rats were sacrificed and collected designated samples (blood, kidney, pancreas) at the 21 days after antioxidant treatment (the 8th week after STZ injection). There was no significant change of body weight during the experiment period in the all groups. The concentration of blood glucose was decreased below 200 mg/dl in MLT. The concentration of HbA$_1$c level was decreased significantly in MLT (7.3$\pm$0.66%) compared with STZ (15.1$\pm$1.58%) (p<0.01). No significant change of SOD activity in the RBC and kidney was found in the all groups. In the pancreas, MLT (1.42$\pm$0.17 U/ml) and AA(1.65$\pm$0.16 U/ml) decreased SOD activity compared with STZ (4.10$\pm$0.27 U/ml). However, there was no significant difference among MLT and AA. The level of malondialdehyde(MDA) as an indicator of lipid peroxydation was decreased in MLT compared with STZ in the plasma, kidney and pancreas(p<0.01). These findings suggest that melatonin could prevent the complications of diabetes mellitus.

Keywords

References

  1. Diabetes v.40 Role of oxidative stress in development of complications in diabetes Baynes JW
  2. Diabetes Care v.14 Vitamine E reduction of protein glycosylation in diabetes Ceriello A;Giugliano D;Quatraro A(et al.)
  3. Ann N Y Acad Sci v.840 Melatonin rhythms in mice:role in autoimmune and lymphoproliferative diseases Conti A;Maestroni GJM
  4. Methods Enzymol v.186 Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal Esterbauer H;Cheeseman KH
  5. Radiol Austriaca v.15 On postoperative irradiation of breast cancer with roentgen-telecobalt Fridrich R
  6. Diabetic care v.19 Oxidative stress and diabetic vascular complications Giugliano D;Ceriello A;Paoulisso G
  7. J Biol Rhythms v.12 Toxicology of melatonin Guardiola, Lemaitre B
  8. J Biol Chem v.261 Nenenzymatic glycation of albumin in vivo(identification of multiple glycosylated) Iberg N;Fluckiger R
  9. Diabetes Res v.6 Vitamin C metabolites and microangiopathy in diabetes mellitus Jennings PE;Chirico S;Jones AF;Lunec J;Barnett AH
  10. Diabetes Res v.7 Serum antioxidant activity in diabetes mellitus Jones AF;Winkles JW;Jennings PE;Florkowski CM;Lunec J;Barnett AH
  11. Diabetes v.46 Targeted overexpression of Cu/Zn siperoxide dismutase protects pancreatic β-cells against oxidative stress Kubisch HM;Wang J;Bray TM;Phillips JP
  12. J Biol Chem v.244 Super oxide dismutase. An enzymatic function for erythrocuprein (hemocuprein) McCord JM;Fridovich I
  13. Drugs v.49 Prospects for the use of antioxidant therapies Maxwell SRJ
  14. Eur J Phamaco v.305 Melatonin affords protection against Kainate-induced in vitro lipid peroxidation in brain Melchorri D;Reiter RJ;Chen LD;Sewerynek E;Nistico G
  15. Anal Biochem v.214 Spectrophotometric assay of superoxide dismutase activity based on the activated autoxidation of a tetracyclic catehol Nebot C;Moutet M;Huet P;Xu JZ;Yadan JC;Chaudiere J
  16. Life Science v.52 Malondialdehide modified protein and their antibodies in the plasma of control and streptozocin induced diabetic rats Lung CC;Pinnas JL;Yahaya MD;Meinke GC;Mooradian AD
  17. J Appl.Physiol v.83 Acute administered melatonin reduces oxidative damage in lung and brain induced by hyperbaric oxygen Pablos MI;Reiter RJ;Chuang JI;Ortiz GG;Guerrero JM;Sewerynek E;Agapito MT;Melchorri D;Lawrence R;Dneke SM
  18. Life Sci v.55 Melatonin: a peroxyl radical scavenger more effective than vitamin E Peieri C;Marra M;Moroni F;Recchioni R;Marcheselli F
  19. Life Science v.56 Protective effect of melatonin on in vivo paraquatinduced oxidative damage in rats Patent GN
  20. Res Commun Chem Pathol Pharmacol v.80 Antioxidant activity of melatonin in mice Pierretich G;Topall G;Courboin G;henriet I.;Laborit H.
  21. Endocrinology v.140 Daily melatonin administration at middle age suppresses male rat visceral fat, plasma leptin. and plasma insulin to youthful levels Rasmussen DD
  22. Ann NY Acad Sci v.719 Melatonin as a free radical scavenger: implications for aging and age-related deseases Reiter RJ;Tan DX;Poeggeler B;Menendez-Pelaez A;Chen LD;Saarela S
  23. Frontiers in Neuroendocrinol v.16 Functional pleiotropy of the neurohormone melatonin: antioxidant protection and neuroendorcine regulation Reiter EJ
  24. J Pineal Res v.18 A review of the evidence supporting melatonin's role as an antioxidant Reiter RJ;Mechiorri D;Sewerynek E;Poeggeler B;Barlow-Walden L;Chuang J;Ortiz GG;Acuna-Castroviejo D.
  25. Neurosci Lett v.226 Melatonin supresses hyperglycemia caused by intracerebroventricular injection of 2-deoxy-D-glucose in rats Shima T;Chun SJ;Nijima A;Bizot-Espiard JG;Guardiola-Lemaitre B;Hosokawa M;Nagai K
  26. Biochem Biophys Res Commun v.197 Biochemical evidence for nitric oxide formation from streptozotocin in isolated pancreatic islets Turk J;Corbett JA;Rhamadham S;Bohrer A;McDaniel ML
  27. Lipid v.26 Vitamine E and the suscepibility of erythrocytes and reconstituted liposomes to oxidative stress in aged diabetics Urano S;Hoshi-Hashizume M;Tochigi N;Matsuo M;Shiraki M;Ito H
  28. Diabetes v.36 no.9 Alterations in free radical tissue-defence mechanisms in streptozotocin-induced diabetes in rat Wohaieb SA;Godin DV
  29. 臨床病理 v.40 糖尿病 フリ-ラジカルと抗酸化機構の變動 朝山光太郞
  30. 臨床病理 v.40 活性酸素 とSOD 片山善章