Pronuclear Formation of Unfertilized or Developmentally-Incompetent Human Oocytes after Different Stimuli in Stimulated IVF Program

미수정란 및 발달부적합 사람난자의 활성화에 관한 연구

  • 전수현 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 정형민 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 신태은 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 고정재 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 이문기 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 차광렬 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 박을순 (서울대학교 농생명공학부 발생공학연구실) ;
  • 장경희 (서울대학교 농생명공학부 발생공학연구실) ;
  • 김태민 (서울대학교 농생명공학부 발생공학연구실)
  • Published : 2001.04.01

Abstract

A total of 92 unfertilized human oocytes were treated with ethanol (EtOH), calcium ionophore A23187 (CI) or electric pulse (EP) for activating pronuclear formation and subsequent development. In Experiment 1, there was a significant (P=0.0001) treatment effect on the activation of unfertilized oocytes. No spontaneous activation was occurred in the control, but activation treatments induced PN formation with various efficacy. More unfertilized oocytes (UFOs) were activated after EtOH or EP treatment than after CI treatment. EP was as effective (63.6 %) as EtOH, but fragmentation was observed in 43% of UFOs activated by EP. Proportion of UFOs that formed presumptive haploid PN (2 PNs+1 PB or 1 PN +2 PBs) was 33.3, 0 and 28.6% after EtOH, CI and EP treatments, respectively. In Experiment 2, a significant (P=0.0362) effect of immature oocytes (IOs) status on activation was fecund. IOs at the GVBD-MI oocytes had higher potential to form PN than those at the GV stage or with abnormal morphology (25 vs. 77.8%). The results of this study clearly demonstrated that the treatment of 10% ethanol for 5 min effectively induced the activation of UFOs. IOs could form pronucleus with high efficacy by ethanol treatment, as long as they grew beyond the GVBD stage.

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