Biotechnology and Bioprocess Engineering:BBE

  • 제6권4호
  • /
  • Pages.306-308
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  • 2001
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Secretory Expression of Human Growth Hormone in Saccharomyces cerevisiae Using Three Different Leader Sequences

  • Hahm, Moon-Sun (Biomolecular Process Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology, P.O, Box115, Yuson, Taejon 305-600) ;
  • Chung, Bong-Hyun (Biomolecular Process Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology, P.O, Box115, Yuson, Taejon 305-600)
  • 발행 : 2001.07.01
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초록

A recombinant human growth hormone(hGH) was expressed as a secretory product in the yeast Saccharomyuces cerevisiae. There different leader sequences derived from the mating fac-tor $\alpha$1(MF$\alpha$1) inulinase and invertase were used to direct the secretion of hGH into the extracel-lular medium. Among three leader sequences tested, the inulinase leader sequence was found to be the most efficient in the secretory expression of hGH. In contrast, no hGH was detected in the ex-tracellular medium with the invertase leader sequence. After 48 h shake-flask culture, the yields of hGH secreted into th emedium by the invertase. MF$\alpha$1 inulinase and invertase leader sequences were approximately 0, 0.3 and 0.9 mg/L, respectively. The secretion efficiencies were also found to be 0, 3.8 and 13% for the invertase , MG$\alpha$1 and inulinase leader sequences, respectively.

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참고문헌

  1. Yeast v.8 Foreign gene expression in yeast: A review Romanos, M. A.;C. A. Scorer;J. J. Clare
  2. Science v.205 Human growth hormone: complementary DNA cloning and expression in bacteria. Martial, J. A.;R. A. Hallewell;J. D. Baxter;H. M. Goodman
  3. Nature v.281 Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone. Goeddel, D. V.;H. L. Heyneker;T. Hozumi;R. Arentzen;K. Itakura;D. G. Yansura;G. Miozzari;R. Crea;P. H. Seeburg
  4. Gene v.55 High-level secretion of human growth hormone by Escherichia coli. Chang, N. C.;M. Rey;B. Bochner;H. Heynecker;G. Grey
  5. DNA v.7 Expression of recombinant growth hormone in Escherichia coli: Effect of the region between the Shine-Dalgarno sequence and the ATG initiation codon. Dalboge, H.;S. Carlsen;E. B. Jensen;T. Christensen;H. M. Dahl
  6. J. Pharm. Biomed. Anal. v.7 Characterization of a secreted form of recombinant derived human growth hormone in Escherichia coli cells. Gellerfors, P.;G. Eketorp;K. Fhulenhag;B. Pavlu;S. Johansson;L. Fryklund
  7. J. Am. Med. Assoc. v.255 Technology spurt resolves growth hormone problem, ends shortage. K. Glasbrenner
  8. Biotechnology v.2 Pseudomonas aeruginosa secretes and correctly processes human growth hormone. Gray, G. L.;K. A. McKeown;A. J. S. Jones;P. H. Seeburg;H. L. Heynecker
  9. Gene v.39 Periplasmic production of correctly processed human growth hormone in Escherichia coli: Natural and bacterial signal sequences are interchangeable. Gray, G. L.;J. S. Baldridge;K. S. McKeown;H. L. Heyneker;C. N. Chang
  10. J. Biotechnol. v.5 Extracellular production of human growth hormone by a head portion of the prepropeptide derived from Bacillus amyloliquefaciens neutral protease in Bacillus subtilis. Nakayama, A.;K. Kawamura;H. Shimada;A. Akaoka;J. Mita;M. Honjo;Y. Furutani
  11. J. Biotechnol. v.62 High-level production of human growth hormone in Escherichia coli by a simple recombinant process. Shin, N. K.;D. Y. Kim;C. S. Shin;M. S. Hong;J. Lee;H. C. Shin
  12. Process Biochem. v.35 High-level secretory production of recombinant human lipocortin-I by Saccharomyces cerevisiae. Chung, B. H.;D. J. Seo;S. W. Nam
  13. J. Bacteriol. v.153 Transformation of intact yeast cells with alkali cations. Ito, H.;Y. Fukuda;K. Murata;A. Kimura
  14. Biotechnol. Bioeng. v.49 Highly efficient secretion of heterologous proteins from Saccharomyces cerevisiae using inulinase signal peptides. Chung, B. H.;S. W. Nam;B. M. Kim;Y. H. Park
  15. J. Microbiol. Biotechnol. v.9 Secretory expression of human αs1-casein in Saccharomyces cerevisiae. Kim, Y. K.;D. Y. Yu;H. A. Kang;S. Yoon;B. H. Chung
  16. Science v.219 Secretion of human interferons by yeast. Hitzeman, R. A.;D. W. Lenny;L. J. Petty;W. J. Kohr;H. L. Levine;D. V. Goeddel
  17. Proc. Natl. Acad. Sci. USA v.81 Expression of human α1-antitrypsin cDNA in the yeast Saccharomyces cerevisiae. Cabezon, T.;M. De Wilde;P. Herion;R. Loriau;A. Bollen
  18. Mol. Cell. Biol. v.4 A deletion that includes the signal peptidase cleavage site impairs processing, glycosylation, and secretion of cell surface yeast acid phosphatase. Haguenauer-Tsapis, R.;A. Hinnen