Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Bioinspired Polymers that Control Intracellular Drug Delivery

  • Allan S. Hoffman (Department of Bioengineering, University of Washington) ;
  • Patrick S. Stayton (Department of Bioengineering, University of Washington) ;
  • Oliver-Press (Fred Hutchinson Cancer Research Center) ;
  • Niren-Murthy (Department of Bioengineering, University of Washington) ;
  • Chantal A. Lackey (Department of Bioengineering, University of Washington) ;
  • Charles-Cheung (Department of Bioengineering, University of Washington) ;
  • Fiona-Black (Department of Bioengineering, University of Washington) ;
  • Jean Campbell (Department of Pathology, University of Washington) ;
  • Nelson Fausto (Department of Pathology, University of Washington) ;
  • Themis R. Kyriakides (Department of Biochemistry, University of Washington) ;
  • Paul-Bornstein (Department of Biochemistry, University of Washington)
  • Published : 2001.07.01
Download PDF
⟨ Previous Next ⟩

Abstract

One of the important characteristics of biological systems os their ability to change im-portant properties in response to small environmental signals. The molecular mechanisms that biological molecules utilize to sense and respond provide interesting models for the development of "smart" polymeric biomaterials with biomimetic properties. An important example of this is the protein coat of viruses, which contains peptide units that facilitate the trafficking of the virus into the cell via endocytosis, then out of the endosome into the cytoplasm, and from there into the nucleus, We have designed a family of synthetic polymers whose compositions have been de-signed to mimic specific peptides on viral coats that facilitate endosomal escape. Our biomimetic polymers are responsive to the lowered pH whinin endosomes, leading to distruption of the en-dosomal membrane and release of important biomolecular druges such as DNA, RNA, peptides and proteins to the cytoplasm before they are trafficked to lysosomes and degraded by lysosomal en-zymes. In this article, we review our work on the design, synthesis and action of such smart, pH-sensitive polymers.

Keywords

References

  1. J. Biol. Chem. v.270 Cellular and molecular barriers to gene transfer by a cationic lipid. Zabner, J.;A. J. Fasbender;T. Moninger;K. A. Poellinger;M. J. Welsh
  2. J. Immunol. v.141 Ricin A-chain containing immunotoxins directed against different epitopes on the CD2 molecule differ in their ability to kill normal and malignant T cells. Press, O. W.;P. J. Martin;P. E. Thorpe;E. S. Vitetta
  3. Physiol. Rev. v.77 Endocytosis. Mukherjee, S.;R. N. Ghosh;F. R. Maxfield
  4. Ann. Rev. Biochem. v.56 The structure and function of the hemagglutinin membrane glycoprotein of influenza virus. Wiley, D. C.;J. J. Skehel
  5. Curr. Biol. v.5 Structural characterization of viral fusion proteins. Hughson, F. M.
  6. Biochem. v.38 Membrane translocation of charged residues at the tips of hydrophobic helices in the T domain of diphtheria toxin Ren, J.;J. C. Sharpe;R. J. Collier;E. London
  7. Proc. Nat. Acad. Sci. USA v.89 Influenza virus hemagglutinin HA-2 Nterminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: Toward a synthetic virus-like gene-transfer vehicle Wagner, E.;C. Plank;K. Zatloukal;M. Cotten;M. L. Birnstiel
  8. J. Biol. Chem. v.269 The influence of endosome-disruptive peptides on gene transfer using synthetic virus-like gene transfer systems. Plank, C.;B. Oberhauser;K. Mechtler;C. Koch;E.Wagner
  9. Bioconj. Chem. v.8 Influence of endosome-destabilizing peptides on efficacy of anti-HIV immunotoxins. Tolstikov, V. V.;R. Cole;H. Fang;S. H. Pincus
  10. Biochem. v.26 pH-Dependent bilayer destabilization by an amphipathic peptide. Subbarao, N. K.;R. A. Parente;F. C. J. Szoka;L. Nadasdi;K. Pongracz
  11. J. Biol. Chem. v.263 pHdependent fusion of phosphatidylcholine small vesicles. Parente, R. A.;S. Nir;F. C. J. Szoka
  12. J. Polymer Sci. Symposium v.51 Structure and properties of pharmacologically-active polymers. Ringsdorf, H.
  13. Acc. Chem. Res. v.25 Polyelectrolytesensitized phospholipid vesicles. Thomas, J. L.;D. A. Tirrell
  14. Biophys. J. v.67 Membrane solubilization by a hydrophobic polyelectrolyte: surface activity and membrane binding. Thomas, J. L.;S. W. Barton;D. A. Tirrell
  15. J. Cont. Rel. v.61 The design and synthesis of polymers for eukaryotic membrane disruption. Murthy, N.;J. R. Robichaud;D. A. Tirrell;P. S. Stayton;A. S. Hoffman
  16. Macromol. Symposia pH-sensitive Hemolysis by Random Copolymers of Alkyl Acrylates and Acrylic Acid. Murthy, N.;I. Chang;P. S. Stayton;A. S. Hoffman
  17. Macromolecules v.34 Fo-cused ultrasound and poly(2-ethylacrylic acid) act syner gistically to disrupt lipid bilayers in vitro. Mourad, P. D.;N. Murthy;T. M. Porter;S. L. Poliachik;L. A. Crum;A. S. Hoffman;P. S. Stayton
  18. Biochim. Biophys. Acta Grafted poly-(ethylene glycol) on lipid surfaces inhibits protein adsorption and cell adhesion. Du, H.;P. Chandaroy;S. W. Hui
  19. J. Cell Biol. v.140 Mice that lack thrombospondin 2 display connective tissue abnormalities that are associated with disordered collagen fibrillogenesis, an increased vascular density, and a bleeding diathesis. Kyriakides, T. R.;Y. H. Zhu;L. T. Smith;S. D. Bain;Z. Yang;M. T. Lin;K. G. Danielson;R. V. Iozzo;M. LaMarca;C. E. McKinney;E. I. Ginns;P. Bornstein
  20. J. Contr. Rel. pH-sensitive polymers that enhance intracellular drug delivery in vivo. Kyriakides, T. R.;C. Y. Cheung;N. Murthy;P. Bornstein;P. S. Stayton;A. S. Hoffman
  21. J. Invest Dermatol. v.113 Accelerated wound healing in mice with a disruption of the thrombospondin 2 gene. Kyriakides, T. R.;J. W. Tam;P. Bornstein
  22. Cancer Res. v.48 Endocytosis and degradation of murine anti-human CD3 monoclonal antibodies by normal and malignant Tlymphocytes. Press, O. W.;J. A. Hansen;A. Farr;P. J. Martin
  23. Cell Immunol. v.137 Intracellular catabolism of radiolabeled anti-CD3 antibodies by leukemic T cells. Geissler, F.;S. K. Anderson;O. Press
  24. Nature v.375 Furin-dependent intracellular activation of the human stromelysin-3 zymogen. Pei, D.;S. J. Weiss
  25. J. Am. Chem. Soc. v.77 The evaluation of inductive and resonance effects on reactivity: I. Hydrolysis rates of acetals and non-conjugated aldehydes and ketones. Taft, R. W.;M. M. Kreevoy