International Journal of Industrial Entomology and Biomaterials
- Volume 3 Issue 1
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- Pages.43-49
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- 2001
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- 3022-7542(pISSN)
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- 2586-4785(eISSN)
Molocular Cloning and Sequence Analysis of the Immediate Early Viral Gene, IE1, from Bombyx mori Nuclear Polyhedrosis Virus K1
- Park, Hye-Jin (College of Natural Resources and Life Science, Dong-A University) ;
- Lee, Kwang-Sik (College of Natural Resources and Life Science, Dong-A University) ;
- Je, Yeon-Ho (School of Agricultural Biotechnology, Seoul National University) ;
- Sohn, Hung-Dae (College of Natural Resources and Life Science, Dong-A University) ;
- Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University)
- Published : 2001.09.01
Abstract
We have cloned and characterized an immediate early-1 gene, iel, which is activated immediately upon entrance of the viral genome into the cell nucleus, from Bombyx mori nuclear polyhedrosis virus (BmNPV) K1 strain. This gene encodes a protein 584 amino acids with a predicted molecular weight of 67 kDa. The promoter and coding regions of BmNPV-K1 ie1 showed high homology with Autographa californica nuclear polyhedrosis virus and BmNPV T3 strain. The BmNPV-K1 ie1 was different from amino acid sequence at 4 positions in BmNPV T3. The location of ie1 gene in the BmNPV-K1 genome was confirmed by Southern blot analysis and its expression patterns at the transcriptional level in the infected cells were confirmed by Nerthern hybridization analysis.