Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Mutagenic Characterization of a Conserved Functional Amino Acid in Fuculose-1-Phosphate Aldolase from Methanococcus jannaschii, a Hyperthermophic Archaea

  • Yoon, Hye-Sook (Department of Chemistry, National Research Laboratory, Hanyang University) ;
  • Kwon, Si-Joong (Department of Chemistry, National Research Laboratory, Hanyang University) ;
  • Han, Myung-Soo (Department of Life Science, Hanyang University) ;
  • Yu, Yeon-Gyu (Structural Biology Center, Korea Institute of Science and Technology) ;
  • Yoon, Moon-Young (Department of Chemistry, National Research Laboratory, Hanyang University)
  • Published : 2001.08.01
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Abstract

To elucidate the putative role of the amido group in the metal binding of the fuculose-1-phosphate aldolase from Methanococcus jannaschii, we have examined a potential targen using site-directed mutagenesis. The replacement of asparagine 25 with leucine or threonine was shown to have a negative effect, not only on catlytic efficiency, but also on substrage recognition as well. The Hill coefficient values yeilded a value of =1. All metals used with the wild-type aldolases exhibited higher activity than that of the mutants. The spectra of the mutants were quite different from the wild-type aldolase. A highly conserved amino acid of asparagine 25 in a related family of aldolase odes not appear to provide sufficient evidence for evolution.

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