Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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In Vitro Detection of Apoptosis in Human Promyleoytic Leukemia HL-60 Cells by $^{1}H$-NMR

  • Lee, Chul-Hoon (Department of Medical Genetics & Institute of Biomedical Science, College of Medicine, Hanyang University) ;
  • Lee, Min-A. (Department of Medical Genetics & Institute of Biomedical Science, College of Medicine, Hanyang University) ;
  • Cho, Youl-Hee (Department of Medical Genetics & Institute of Biomedical Science, College of Medicine, Hanyang University) ;
  • Lim, Hae-Young (Department of Medical Genetics & Institute of Biomedical Science, College of Medicine, Hanyang University) ;
  • Jung, Ji-Hyun (Department of Applied Biology & Chemistry, Konkuk University) ;
  • Kim, Kyung H. (Graduate School of Biotechnology, Kora University) ;
  • Lim, Yoong-Ho (Department of Applied Biology & Chemistry, Konkuk University)
  • Published : 2001.06.01
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Abstract

$^1H$-NMR spectroscopy was used to detect apoptosis in HL-60 cells in vitro. The relationship between cell apoptosis and NMR data was validated by the flow cytometry assay. To evaluate the NMR apoptosis results, the ratio of methylene and methyl groups caused by lipids was used. In addition, an identical analysis was applied to HepG2 cells. Detection of apoptotic cell death by NMR spectroscopy was oserved.

Keywords

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