Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

The Phylogenetic Relationship of Several Oscillatorian Cyanobacteria, Forming Blooms at Daecheong Reservoirs, Based on Partial 16S rRNA Gene Sequences

  • Lee, Wook-Jae (Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology) ;
  • Bae, Kyung-Sook (Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2001.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The partial 16S gene sequences of six filamentous cyanobacterial strains, Oscillatoria lmosa KCTC AG10168, Oscillatoria princeps KCTC AG10153, Oscillatoria sp. KCTC AG 10184, Phormidium tenue KCTC AG10158, Phormidium parchydematicum KCTC AG10164, and Lyngbya hieronymusii KCTC AG10199, which were isolated in the late summer at Daecheong Reservoirs, were determined and assigned their phylogenetic and taxonomic position among taxa of order Ocillatoriales whose partial 16S rRNA gene sequences aligned in this suty, were very heterogeneously clustered with other taxa. The two strains, Oscillatoria limosa KCTC AG10168 and O. princeps KCTC AG10153, formed a cluster with O. sancta PCC7515, which supported 64% of the bootstrap trees with high similarity (19-96.15%). Strain Oscillatoria sp. KCTC AG10184, that was known to produce a nasty substance, was closely related to the toxic Oscillatoria group. The study on morphological variation in various environments and toxin production will confirm the taxonomic status of these species. Phormidium tenus KCTC AG10158 and Phormidium parchydematicum KCTC AG10164 made a cluster with other oscillatorian species of Phormidium, Oscillatoria, and Leptolynbya, which supproted 100% of the bootstrap trees with a very high sequence smilarity (96.8-99.8%) in thsi study. The sequence analysis in this study also supported that taxa of oscillatoriales are not monophyletic. Some of the fractures, such as the presence or absence of sheath and cell shape, which were used to define them, would be inadequate and should be reconfirmed. We suggest that sequences of partial 16S rRNA gene fragments aligned in this study should be more useful than morphological features in the identification and reconfirmation of the taxonomic status of these oscillactorian cyanobacteria.

Keywords

References

  1. Bergey's Manual of Systematic Bacteriology v.3 Subsection Ⅲ. Order Oscillatoriales Castenholz, R. E.;J. B. Waterbury;Staley, J. T.(ed.);M. P. Bryant(ed.);N. Pfeng(ed.);J. G. Holt(ed.)
  2. Bergey's Manual of Systematic Bacteriology v.3 Taxa of the Cyanobacteria Castenholz, R. E.;J. B. Waterbury;Staley, J. T.(ed.);M. P. Bryant(ed.);N. Pfeng(ed.);J. G. Holt(ed.)
  3. Ph. D. Thesis. Computer-assisted classification and identification of actinomycetes Chun, J.
  4. Korean J. Environ. Biol. v.13 A study on the phytoplankton community in Dae-Cheong lake Chung, S. J.
  5. Evolution v.39 Confidence limits on phylogenies: An approach using the bootstrap Felsenstein, J.
  6. PHYLIP (Phylogenetic inference package)(version 3.5c) Felsenstein, J.
  7. J. Bacteriol. v.170 Evolutionary relationships among cyanobacteria and green chloroplasts Giovannoni, S. J.;S. Turner;G. J. Barns;D. J. Lane;N. R. Pace
  8. J. Gen. Appl. Microbiol. v.43 Phylogenetic relationships of filamentous cyanobacterial taxa inferred from 16S rRNA sequence divergence Ishida, T.;A. Yokota;J. Sugiyama
  9. J. Mol. Evol. v.16 A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences Kimura, M.
  10. J. Microbiol. Biotechnol. v.6 Nucleotide sequence of 16S rRNA gene from Streptomyces melanosporofaciens 7489 Lee, D. S.;S. O. Suh;S. K. Hwang;T. K. Kwon;T. H. Kim;W. C. Shin;S. D. Hong
  11. Korean J. Limnol. v.32 Dynamics of phytoplankton community in Lake Daechung Lee, J. H.
  12. Syst. Appl. Microbiol. v.17 Phylogenetic relationships among filamentous helical cyanobacteria investigated on the basis of 16S ribosomal RNA gene sequence analysis Nelissen, B.;A. Willmontte;J.-M. Neefs;R. De Wachter
  13. J. Mol. Evol. v.42 Phylogenetic relationships of nonaxenic filamentous cyanobacterial strains based on 16S rRNA sequence analysis Nelissen, B.;R. De Baere;A. Willmontte;R. De Wachter
  14. Appl. Environ. Microbiol. v.63 PCR primers to amplify 16S rRNA genes from cyanobacteria Nubel, U.;F. Garcia-Pichel;G. Muyzer
  15. ACBIOS v.12 TREEVIEW: An application to display phylogenetic trees on personal computers Page, R. D. M.
  16. J. Microbiol. Biotechnol. v.10 Services of algae to the environment Rai, L. C.;H. D. Kumar;F. H. Mohn;C. J. Soeder
  17. Arch Hydrobiol.;Algal Studies v.38;39 no.SUP.71 Planktonic species of Oscillatoria (Cyanophyceae) from Norway Skulberg, O. M.;R. Skulberg
  18. J. Phycol. v.33 Molecular systematics of Ectocarpus and Kuckuckia (Ectocarpales, Paheophyceae) inferred from phylogenetic analysis of nuclear- and plastid-encoded DNA sequences Stache-Crain, B.;D. G. Muller;L. J. Goff
  19. Phylogenetic Analysis Using Parsimony(Version 4) PAUP Swofford, D. L.
  20. J. Microbiol. Biotechnol. v.9 Cyanobacterial toxins: The current status Tyagi, M. B.;J. K. Thakur;D. P. Singh;A. Kumar;E.G. Prasuna;A. Kumar
  21. J. Phycol. v.28 Taxonomic study of marine oscillatoriacean strains (cyanobacteria) with narrow trichomes. Ⅱ. Nucleotide sequence analysis of the 16S ribosomal RNA Wilmontte, A.;S. Turner;Y. Van de Peer;N. R. Pace
  22. 16S ribosomal RNA sequence analysis Microbiology v.140 Evolutionary affiliation of the marine nitrogen fixing cyanobacterium Trichosedmium sp. strain NIBB 1067 Wilmontte, A.;J.-M. Neefs;R. De Wachter
  23. Microbiol. Rev. v.51 Bacterial evolution Woese, C. R.
  24. J. Microbiol. Biotechnol. v.8 Isolation of novel alkalophilic Bacillus alcalophilus subsp. YB380 and the characteristics of its yeast cell wall hydrolase Yeo, I. H.;S. K. Han;J. H. Yu;D. H. Bai