Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of Collagen Concentration on the Viability and Metabolic Function of Encapsulated Hepatocytes

  • Kim, Sung-Koo (Division of Food and Biotechnology, Pukyong National University) ;
  • Yu, Sun-Hee (Division of Food and Biotechnology, Pukyong National University) ;
  • Lee, Ji-Hyun (Division of Food and Biotechnology, Pukyong National University) ;
  • Axel Racemacher (Division of Food and Biotechnology, Pukyong National University) ;
  • Lee, Doo-Hoon (Institut fur Biotechnologie, Technische Universitat Berlin) ;
  • Park, Jung-Keug
  • Published : 2001.06.01
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Abstract

Chitosan/alginate capsules were formed by electrostatic interactions and had appropriated mechanical strength, permeability to albumin, and stability to hepatocytes. Rat hepatocytes were isolated and immobilized in chitosan/alginate capsules. During the encapsulation process with hepatocyte, 10% of viability was decreased mainly due to the low pH of the chitosan solution. Among various capsule fabrication methods, the chitosan-alginate capsule showed the highest mechanical strength. Addition of collagen in the capsule with hepatocytes enhanced hepatic metabolism as well as the cell viability for 2 weeks of culture. The hepatocyte in the capsule without collagen decreased the viability to 10% for 2-week cultures.

Keywords

References

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