Enzymatic Degradation of Poly(${\gamma}$-glutamic acid) Hydrogel Prepared by ${\gamma}$-Ray Irradiation

  • Hara, Toshio (Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University) ;
  • Choi, Seong-Hyun (Department of Agricultural Chemistry, Chungnam National University) ;
  • Choi, Woo-Young (Department of Agricultural Chemistry, Chungnam National University)
  • 발행 : 2001.04.01

초록

A bacterial strain PH-4, which produces an enzyme catalyzing the degradation of crosslinked poly(${\gamma}$-glutamic acid) hydrogels, was isolated and identified as a Flavobacterium sp. The enzyme was obtained by the sonication of the bacterial cells preincubated in a Bouillon medium with shaking, without adding of poly(${\gamma}$-glutamic acid) as an inducer. The products of the hydrogel degraded by the crude enzyme agreed closely with the depolymerized materials in SDS-polyacrylamide gel electrophoresis using methylene blue staining, and with a glutamic acid monomer on thin-layer chromatography, thereby suggesting that strain PH-4 produced a kind of exohydrolase.

키워드

참고문헌

  1. J. Environ. Poly. Degrad. v.4 Hydrolytic and enzymatic degradation of poly(γ-glutamic acid) hydrogels and their application in slow-release systems for proteins Fan, K.;D. Gonzales;M. Scvoian
  2. Biosci. Biotech. Biochem. v.56 Biosynthesis and hydrolysis of poly(γ-glutamic acid) from Bacillus subtilis IFO 3335 Goto, A.;M. Kunioka
  3. J. Bacteriol. v.180 Variation in flagellin genes and proteins of Bukholderia cepacia Hales, B. A.;A. W. Morgan;C. A. Hart;C. Winstanley
  4. Radioisotopes Synthesis of super water absorbent prepared from Natto string by γ-ray irradiation Hara, T.
  5. Kyu-dai Kobo v.2 Synthesis and development of natto resign prepared from natto string Hara, T.
  6. Chemistry and Chemical Industry v.52 Synthesis of super water absorbent prepared from Natto string with epoxide Hara, T.
  7. J. Appl. Bacteriol. v.43 A taxonomic study of Flavobacteria and related Gram negative yellow pigmented rods Hayes, P. R.
  8. J. Lab. Clin. Med. v.44 Two simple media for the demonstration of pyocyanin and fluorescin King, E. O.;M. K. Ward;D. E. Raney
  9. Biosci. Biotech. Biochem. v.57 Production of poly(γ-glutamic acid) by Bacillus subtilis F-2-01 Kubota, H.;T. Matsunobu;K. Uotani;H. Takebe;A. Satoh;T. Tanaka;M. Taniguchi
  10. Kobunshi Ronbunshu v.50 Properties of hydrogels prepared by γ-irradiation in microbial poly(γ-glutamic acid) aqueous solutions Kunioka, M.
  11. Curr. Microbiol. v.4 Taxonomy of nonfermentative bacilli: the Ⅱ K-2 group Levine, M. G.;M. J. Pickett;M. Mandel
  12. Nippon Shokuhin Kogyo Gakkaishi v.35 Screening of koji mold with hydrolytic activity on γ-polyglutamic acid Ueda, S.;R. Ohba;S. Ijiri
  13. J. Bacteriol. v.74 A new species (Flavobacterium polyglutamicum) which hydrolyzes the γ-L-glutamyl bond in polypeptides Volcani, B. E.;P. Margalith
  14. Int. J. Syst. Bacteriol. v.29 Flavobacterium aquatile and Flavobacterium meningosepticum: Glucose nonfermenters with similar flagella morphologies Webster, J. A.;R. Hugh
  15. J. Bacteriol. v.69 Flavobacterium aquatile (Frankland and frankland) Bergey et al., type species of the genus Flavobacterium Weeks, O. B.
  16. Biosci. Biotech. Biochem. v.60 Detection of γ-polyglutamic acid (γ-PGA) by SDS-PAGE Yanaguchi, F.;Y. Ogawa;M. Kikuchi;K. Yuasa;H. Motai